| Objective: Malignant tumor has become a serious impact on the healthand life of human factors. At present the whole world every year millions ofpeople dying of cancer.While the incidence and fatality rate rises year byyear.Cure rates have not improved.The patients died formn colorectal cancerpatients accounted for about1000000cases.Colorectal cancer as the mostcommon cancer in gastrointestinal tumors,there are950thousand cases newcases each year worldwide,nearly40million the number of new cases is inChina.Colorectal cancer situation has become very serious.Domestic andforeign scholars on the pathogenesis of colorectal cancer has been widelydiscussed,but current colorectal cancer on the exact pathogenesis is still notclear.With wide application of molecular biology,colorectal cancer moleculargenetics characteristics gradually to be understood.At present, has confirmedthat the incidence of colorectal cancer is a gene, multi-factor, multi-stepprocess.TIP30is discovered when Xiao H studying of human immunodeficiencyvirus In vitro transcription.It is a30ku Tat Binding protein also known asHTATIP2.TIP30is discovered when Xiao H studying of humanimmunodeficiency virus In vitro transcription.It is a30ku Tat Binding proteinalso known as HTATIP2.TIP30as HIV transcription cofactors can interactwith Tat binding proteins to increase HIV value.TIP30and CC3tumorsuppressor gene are the same protein by sequence analysis.CC3is a genewhich can inhibit tumor metastasis,and it was found by Shtivelman E[2] usingof RNA differential display technique. CC3is high expression in The lowmetastatic classic small cell lung carcinoma(NCIH345,NCIH69,NCI146),butit is no expression in variant small cell lung carcinoma(H82,N417).RASSF1A is one of the RASSF1different versions (at least6) in versions of the most widely researched.There are16CpG locis in the promoter regionof RASSF1A.The length of promoter region's cDNA is1873bp, and contains2promoter region and6exons(1α,2αβ,3,4,5,6).The different degree ofmethylation of RASSF1A gene leads to reduced expression or deletion andhas closely related with human solid tumors. RASSF1A gene in normallung,stomach,colorectal,cervical and other organizations has a wide expression,but in lung cancer, gastric cancer,colorectal cancer,cervical cancer,postoperative specimens and tumor cell lines showes low expression or veryhigh loss rate.Abnormalities of RASSF1A gene methylation and tumourformation has a very close relationship, with the study and development ofepigenetics.The experiment based on colorectal carcinoma tissues, adjacent tissues,normal colorectal mucosa tissue in TIP30and RASSF1A detection, so as toexplore the relationship between TIP30and RASSF1A.This experiment to explore TIP30and RASSF1A in colorectal cancer,the development of the role and relationship with clinical and pathologicalcharacteristics and provides diagnosis, treatment for colorectal cancer.Methods: Immunohistochemical(streptavidin-peroxidase, SP) techniquewas applied to detected the expression of TIP30and in50cases of colorectalcarcinoma,50cases of the proximal adjacent tissues and20cases of normallarge intestinal mucosa,and the comprehensive analysis of combined with thepatient's age,gender,infiltration degree,lymph node metastasisclinicopathological factors and so on. Using the statistical software SPSS13.0statistical data processing, count with χ2test, P<0.05is statisticallysignificant.It analysed the relationship between the expression of both inColorectal cancer by using Spearman rank corrdlationg which is one ofnon-parameter statistics.Reagents used:mouse anti-human monoclonal antibody was purchasedfrom SANTA CRUZ company,its working concentration is1:300,mouseanti-human RASSF1A antibody was purchased from SANTA CRUZcompany,its working concentration is1:300. SP immunohistochemistry kit was purchased from Beijing Boao Sen Corporation, DAB color reagent kitwas purchased from Beijing Zhongshan Golden Bridge Biotechnology Co.,Ltd..Results:1The expression of TIP30and RASSF1A in Colorectal cancer tissues,adjacent tissues and control tissues1.1TIP30showed high expression in normal colorectal tissue,the positiveexpression rate of95%(19/20),adjacent tissues, the positive expression rateof70%(35/50), expressed in colorectal cancer was13%(6/50).The positiveexpression rate of TIP30in normal colorectal mucosa was significantly higherthan the adjacent normal tissues of colorectal cancer,but there was nosignificant difference(P>0.05).Its positive expression in the organization nextto the colorectal cancer rate is higher than in colorectal carcinoma, resultingin significant differences (P <0.05).1.2RASSF1A expression in normal colorectal tissue is high,the positive ratewas90%(18/20),the positive expression rate was72%(36/50) in the adjacenttissues,expressed in colorectal cancer was16%(8/50).RASSF1A expression innormal colorectal mucosa was significantly higher than the adjacent normaltissues of colorectal cancer,but the difference was not significant (P>0.05),next to the colorectal cancer tissues was significantly higher than in colorectalcarcinoma and the difference was significant (P <0.01).2The relationship between TIP30and RASSF1A protein expression andclinicopathologic features in colorectal cancer tissues2.1TIP30protein expression in colorectal cancer tissue with the patient's age,gender had no correlation(P>0.05).TIP30expression in colorectal carcinomawithout lymph node metastasis was71.4%(20/28),significantly higher thanthe lymph node metastasis of colorectal carcinoma in31.8%(7/22),thedifference was statistically significant (P<0.01).TIP30expression in colorectalcancer not invading serosa organization was60%(18/30),significantly higherthan the expression of colorectal cancer invasion and serosal organization rateof25%(5/20),the difference was statistically significant(P<0.05).TIP30 expression in high grade colorectal carcinoma was63.2%(24/38), significantlyhigher than the poorly differentiated tissue of colorectal cancer expression rateof16.7%(2/12), the difference was statistically significant (P <0.01).2.2RASSF1A protein expression had no correlation with patient age, gender(P>0.05).RASSF1A expression in colorectal carcinoma without lymph nodemetastasis was75%(21/28),significantly higher than the lymph nodemetastasis of colorectal carcinoma in27.3%(6/22),the difference wasstatistically significant (P<0.01).RASSF1A expression in colorectal cancer notinvading serosa organization was66.7%(20/30),significantly higher than theinvasion and serosal tissue expression of colorectal cancer rate25%(5/20), thedifference was statistically significant (P<0.01).TIP30expression in highgrade colorectal carcinoma was68.4%(26/38),significantly higher than thepoorly differentiated organization of colorectal cancer expression rate of33.3%(4/12),the difference was statistically significant (P <0.05).3The relationship between TIP30and RASSF1A expression in colorectalcarcinomaUsing the Spearman rank correlation analysis, the results of TIP30andRASSF1A expression was positively correlated.(rs=0.292, P <0.01)Conclusions:(1)TIP30protein expression in colorectal cancer tissue hasno correlation with the patient's age,gender.TIP30protein was highlyexpressed in normal colorectal mucosa and was closely related with lymphnode metastasis,depth of invasion,degree of differentiation.TIP30proteinexpression in colorectal cancer without lymph node metastasis is higher thanthe lymph node metastasis in colorectal carcinoma.Not invasive serousmembrane organization of colorectal cancer was significantly higher than theexpression in invasive serous membrane organization of colorectal cancer.TIP30protein expression in high grade colorectal carcinoma was significantlyhigher than the poorly differentiated colorectal cancer tissues.(2) RASSF1Aprotein expression in colorectal cancer tissue had no correlation with thepatient's age,gender.RASSF1A protein was highly expressed in normalcolorectal mucosa and has closely related with lymph node metastasis,depth of invasion,degree of differentiation. RASSF1A protein expression in colorectalcancer without lymph node metastasis is higher than the tissue with lymphnode metastasis.Not invasive serous membrane organization of colorectalcancer was significantly higher than the expression in invasive serousmembrane organization of colorectal cancer.RASSF1A protein expression inhigh grade colorectal carcinoma was significantly higher than in poorlydifferentiated colorectal cancer tissue expression.(3) TIP30and RASSF1Aexpression in colorectal cancer tissues was positively correlated,both of themplay an important role in colorectal cancer occurrence and development.TIP30and RASSF1A may closely related to occur,and development,invasion,metastasis and prognosis of colorectal cancer and the joint evaluation of itsfunction has important clinical significance. |
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