Relationship Between TNFSF15Single Nucleotide Polymorphisms And Ulcerative Colitis

Ulcerative colitis (UC) is a group of chronic non-specific inflammatorydisease, mainly involving the rectum and colon, whose etiology is stillunknown. The generic term for UC and Crohn's disease (CD) is inflammatorybowel disease (IBD). The pathogenesis of UC can be summarized that on thebasis of environmental factors on genetic susceptibility, intestines produce theimmune mechanism and non-immune mechanism with participation of theintestinal flora, eventually occurring intestinal immune response andinflammation. In recent years, many studies have shown that the incidence ofUC is closely related to genes, and tumor necrosis factor superfamily member15(TNFSF15) gene becomes one of the hot. Tumor necrosis factor-like ligand1A (TL1A), protein product of TNFSF15can active T cells, and promotinflammatory cytokine secretion, which plays an important role in immuneregulation and inflammatory diseases.Objective: To investigate the relationship between the UC geneticsusceptibility and eight TNFSF15single nucleotide polymorphisms (SNPs)(rs11554257, rs3810936, rs4263839, rs4979462, rs6478108, rs6478109,rs7848647and rs7865494).Methods:71Han Chinese patients with UC, diagnosed in the SecondHospital of Hebei Medical University, were selected as the case group, and71healthy check-ups as the control group matched in age and gender. TNFSF15SNPs were detected by the matrix-assisted laser desorption/ionizationtime-of-flight mass spectrometry (MALDI-TOF MS) technology, andenotypyed by MassARRAY Typer software system. χ2test or Fisher's exacttest were used to perform Weinberg Equilibrium tests of the genotypefrequencies among cases and controls. For the case-control analysis,comparisons of genotypes and allele frequencies were also performed, where appropriate. And the association between TNFSF15SNPs and the clinicalcharacteristics of UC was analyzed by this means. Statistical analysis wasperformed using SPSS software version13.0.Results: None of the eight TNFSF15SNPs (rs11554257, rs3810936,rs4263839, rs4979462, rs6478108, rs6478109, rs7848647and rs7865494)showed significant deviations from Hardy-Weinberg equilibrium(P>0.05). Sothe samples have group representative. The genotypic and allelic distributionsof eight SNPs among UC patients and controls failed to show any significantmarkers (P values in turn were1.00,1.00,0.47,0.70,0.40,0.40,1.00and0.15in genotype frequencise; P values in turn were0.86,0.94,0.73,0.69,0.68,0.68,0.68and0.14in allele frequencise). Upon association analyses bygender, two markers from TNFSF15showed marginally significantassociation with UC in men only (rs3810936P=0.02, rs4979462P=0.01),especially genotype CT of rs3810936and genotype CC of rs4979462.However, this significance was not confirmed after Bonfferoni correction(rs3810936Pc=0.16, rs4979462Pc=0.08). And there is no significantcorrelation between allele frequencies of eight SNPs and UC clinical features(P>0.05).Conclusion:1The SNPs of TNFSF15(rs11554257, rs3810936, rs4263839,rs4979462, rs6478108, rs6478109, rs7848647and rs7865494) in Chinese Hanpeople were not related to UC genetic susceptibility.2The SNPs of TNFSF15(rs11554257, rs3810936, rs4263839,rs4979462, rs6478108, rs6478109, rs7848647and rs7865494) in Chinese Hanpeople were not associated with clinical features of UC.