| Objective:To study the effect of FATl0down-regulation by small interfering RNAinterference on the invasion and migration of hepatoma carcinoma cell linesMHCC97H and Huh-7.Methods:1.The siRNA of FAT10gene was transiently transfected into MHCC97H andHuh-7cells by LipofectamineTM2000, The FAT10mRNA and protein expressionwas detected by RT-PCR,Real Time PCR and Western Blot.2. To detect the influence on biological behavior of MHCC97H and Huh-7aftertransfection. The effect on cell invasion and migration was detected by,wound—healing assay and Matrigel invasion assay when FAT10was degraded bysiRNA.Results:1. The expression of FAT10mRNA and protein was degraded obviously bythe detection of RT-PCR,Real Time PCR and Western Blot.2. The invasiveness and motility of treated MHCC97H and Huh-7cells wereinhibited in vitro while both FATl0mRNA and protein expression were significantlydecreased in the experimental group compared with controls.Conclusion:The invasion and motility of MHCC97H and Huh-7cells were significantlyinhibited when both FATl0mRNA and protein expression were significantlydecreased by small interfering RNA interference. FAT10may play an important rolein the process of hepatoma carcinoma cell invasion and metastasis. |
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