FAT10Regulate MMP2Expression On Invasion And Metastasis In Primary Hepatocellular Carcinoma

Objective:To study the effect of FATl0down-regulation by small interfering RNAinterference on the invasion and migration of hepatoma carcinoma cell linesMHCC97H and Huh-7through matrix metalloproteinase2Methods:1.The siRNA of FAT10gene was transiently transfected into MHCC97H andHuh-7cells by LipofectamineTM2000, expression of The FAT10mRNA and proteinwas detected by realtime fluorescence quantitative PCR and Western Blot.2.The expression of MMP2protein and mRNA was detected for MHCC97H andHuh-7cells by ealtime fluorescence quantitative PCR and Western Blot.3. To detect the influence on biological behavior of MHCC97H and Huh-7aftertransfection. The effect on cell invasion and migration was detected by wound-healing assay and Transwell Matrigel invasion assayResults:1. Realtime fluorescence quantitative PCR and Western Blot results found thatthe expression of FAT10mRNA and proein was decreased obviously when the siRNAof FAT10gene was transiently transfected into MHCC97H and Huh-7cells byLipofectamine TM2000.2. Knockdown the expression of FAT10,the expression of MMP2mRNA andprotein also decreased3. The invasiveness and motility of treated MHCC97H and Huh-7cells wereinhibited in vitro while both FATl0mRNA and protein expression were significantlydecreased in the experimental group compared with controls.Conclusion:when both FATl0mRNA and protein expression were significantly decreased bysmall interfering RNA interference. MMP2mRNA and protein expression was alsodegraded obviously.The invasion and motility of MHCC97H and Huh-7cells weresignificantly inhibited.FAT10may play an important role in the process of hepatoma carcinoma cell invasion and metastasis through matrix metalloproteinase2.