Effect Of Targeted Interference Protein 73 Gene On Invasion And Metastasis Of Hepatocellular Carcinoma Cells And Molecular Mechanisms

Objective:To investigate the effect of targeting interference the Golgi protein 73 (Golgi glycoprotein 73, GP73) gene on the migration and invasion abilities of hepatoma SMMC-7721 and Bel-7404 cell.Methods by siRNA liposome-mediated transfection the SMMC-7721 and Bel-7404 cells and divided into 3 groups:control group (SMMC-7721 and Bel-7404 cells), negative control group (Transfection random sequence siRNA) and the experimental group (transfected GP73 siRNA). Quantitative PCR (real-time polymerase chain reaction, Real-time Polymerase Chain Reaction, called RT-PCR) was used to measure the expression of GP73 genes after targeted interference of GP73 gene; Transwell and cell scratch assay experimental determination of each group cell invasion and migration ability. Results The GP73 mRNA expression and protein levels were significantly lower than non-transfected group and the control group, the difference was statistically significant (P<0.05). Transwell chamber experiments and cell scratch assay show interference GP73 expression on SMMC-7721 and Bel-7404 cells the invasion and migration ability were significant inhibition (P<0.05). Conclusion GP73 gene can promote the hepatoma cells invasion and metastasis. GP73 gene is expected to be a potential target for suppress the invasion and metastasis of liver cancer.