Study On Toxicological Effect Of Environmental Estrogens Nonylphenol On The Bisexual Artemia

Environmental estrogens (endocrine disrupting chemicals, EDC) as exogenouschemicals can disrupt the normal endocrine function, development, reproduction andbehavior of organisms. Nonylphenol (NP), being resulted primarily from the degradationof nonylphenol ethoxylates (or NPEOs), is a typical environmental estrogens and hasbeen regarded as one of priority pollutants in the blacklist. Aquatic organisms, foodsecurity, and ecological stability can be easily influenced by EDC. In the present paper, aseries of ecological experiments of toxicological effect of environmental estrogensnonylphenol on the bisexual Artemia were carried out to discuss the response of Artemiapopulation. The results are summarized as follows:1. Dynamical effect of NP on Artemia populationToxicity of NP to Artemia population was studied through chronic toxicityexperiment. The results represented that survival rate of higher level concentrations(300~600μg/L) were significantly lower than that of control, and survival rate of adulthas no significant difference between the test concentrations and the control. It wasshown that NP higher than 300μg/L could threaten the nauplius. During adult stagesurvival, the rate of female Artemia with egg capsule of test concentrations was lowerthan that of control. It was shown that NP could affect the development of femaleArtemia. The reproduction experiments showed that that survival rate of offspring beforeadult stage in test concentrations was significantly lower than that in the control, and itwas speculate that the resistance of the offspring reproduced from adult Artemia ofchronic toxicity experiment could become lower than their parental generation.2. The effect of NP on growth and reproduction of ArtemiaResults of observation and measurement of the whole life cycle of F1 generation andparental generation of Artemia showed that the growth rate of larvas in higer levelconcentrations was stunted by NP estrogen. And during the extension of exposure time,the growth rate of Artemia in the whole life cycle in each test concentration was allinhibited. as well as individual body length of parental generation and F1 generation were shorter than that in the control respectively. It was shown that NP could inhibit thedevelopment of Artemia. Moreover, isolated culture test of adult Artemia showed that theoviparity rate and malformation rate in high level concentrations was significantly higherthan that in the control. It was shown that NP could result the egg of Artemia inabnormality.