Single Cell Oil Production From Hydrolysates Of Inulin By Trichosporon Cutaneum

Biodiesel as a renewable alternative energy sources, due to its outstandingenvironmental protection was recognized as green alternatives for fossil fuels,and has brought high attention around the world. Microbial oil grease is a newresource of oil. Some cheap carbon source used to produce microbial oil hasbecome a hot topic.In this paper, using the inulin which extracted from Jerusalem artichoke as acarbon source and Trichosporon cutaneum as oil production strains, thefermentation grease capacity of T. cutaneum was studied, and its conditions offermentation were optimized. Directly fermentation of Jerusalem artichokepowder extract in oil production by T. cutaneum was also studied. The mainresults are as follows:(1) A higher oil yields were up to30%by T. cutaneum fermented inulin. Bygas chromatography, the fatty acid compositions were mainly of the C16and C18series fatty acids, and in which more than80.5%were C16:0, C18:1and C18:2,especially C18:1up to53.1%. The composition is similar to plant oil.(2) The effect of enzyme and acid hydrolysis of inulin on T. cutaneumfermented inulin producing oil were investigated. The results showed that theinulinase decomposition inulin was better than acid solution and obtained ahigher biomass and oil content. The biomass and grease content achieved12.02g/L and4.34g/L respectively when the inulin concentration was60g/L,inulinase added was80U/50mL fermentation liquid, first at55℃insulation andenzymatic8h, and then access T. cutaneum, at30℃, fermentation72h.(3) Using single factor experiment, Plaekett-Burman design and responsesurface analysis method, the medium of T. cutaneum fermented inulin producedoil were optimized. The best ratio of culture medium was (g/L): inulin60.0, yeastextract6.55, calcium chloride0.42, zinc sulfate0.342, peptone2.0, magnesium sulfate0.5, potassium dihydrogen phosphate1.0and manganese chloride0.3.Under the optimization of culture conditions, at30℃, fermentation72h, thebiomass and grease content reached13.56g/L and5.34g/L respectively. Thegrease content increased11.2%than before optimization.(4) The extraction conditions of inulin from Jerusalem artichoke powderwere optimized through the single factor and response surface analysis. Theoptimum extraction conditions were: Jerusalem artichoke powder concentrationof56.9g/L, temperature82.2℃,89.45min. The inulin extraction rate was98.9%.The biomass and grease content with T. cutaneum direct fermentation extract ofJerusalem artichoke powder is higher than with inulin as raw materialfermentation cultures, and the biomass and grease content received14.25g/L and5.59g/L respectively. These results showed that Jerusalem artichoke powdercould be used as the materials of T. cutaneum fermentation to produce oil.