The Research Of Combined Biological And Ecological Repair On Black And Odorous River Pollution Treatment

Withing the rapid development of economy and society,a large number of sewage,wastewater and solid waste were discharged into the river, causing the serious pollutionof river water, resulting in black smelly water. It made enormous ecological destructionand economic loss, seriously affectted the life and physical health ofresidents.Accordingly,it is significant that study of black and odorous river pollutiontreatment and its control is very urgent. Basis on collecting literature data and multiplesite investigation,it has been put foward that ecological revetment+aeration+suspensionfillers+dosing microbial agents biological-ecological combined in situ remediationtechnology in which microbial remediation is key, the technology was used theblack-odor pollution river.There are a lot of factors which cause the change of black stinking river,determined the key influencing factors of change is essential.Selected the Liangtan riveras the research object, color illustrates the value of （CH） for black and odorous index,DO, COD, NH₃-N, TP, Fe, BOD and pH were studied for correlation of black andodorous water. The results show that CH and DO, COD and NH₃-N have bettercorrelation, Where DO negative correlation,DO, COD and NH₃-N as the effect of blackand odorous water important factor.Microbial agents is the core of bioremediation technology, the research group get12stains which has efficient degradation of the black-odor water from the nature ofwater and sediment,of which the1,2,4,5,7,13strains have a high COD degradationeffects, degradation rate close to or more than69%;10,17,18strains there is a strongdegradation ability of ammonia,degradation rate approaching or more than32%;28,40,41strains have strong degradation of TP,degradation rate of close to or morethan64%. By morphological observation and16S rDNA identification of strains,12strains belonging to Bacillus subtilis, Chryseobacterium sp., Acinetobacter sp.,Psychrobacter sp., Arcobacter sp., Pseudomonas sp, Shewanella sp., Aeromonas sp.,andRalstonia sp., ensured the diversity of the species. The results of strains the trend ofoxygen show that1,2,7,10,13,17,40strains is erobic bacteria,4,5,18,28,41strains isfacultative bacteria.1,2,4,5,7,13strains were combined CQ1,10,17,18strains werecombined CQ2,28,40,41strains were combined CQ3, and the three mixed bacteriawere compounded and determined their ratio, the results show that the combination of the best degradation performance of the CQ1, CQ2, CQ3mixed bacteria, the best strainratio is2:2:1.The conditions of growth and degradation for compound bacterium were studied,the compound bacterium of the best growing conditions are: temperature28-34℃,initial pH7-8.5, shaking speed160-220r/min, compound bacterium culture25h ofliving bacteria reached the maximum, as the best time of inoculation; Best degradationcondition for compound bacterium to remove the pollutants is that ratio of0.5%,temperature25℃, pH6-9, shaking speed180r/min, the optimal degradation range ofCOD is200-60mg/L, the optimal degradation range of NH₃-N is60-15mg/L, theoptimal degradation range of TP is120-30mg/L. Under these conditions, compoundbacterium to black-odor wastewater COD, NH₃-N and TP degradation rate reached90%,36%and76%. Sediment degradation experiments showed that compound bacterium onriver sediment has a certain degradation ability.“Ecological revetment+aeration+suspension fillers+dosing microbialagents”biological-ecological combined in situ remediation technology was applied inliangtan River of Zhenwu tributaries black-odor pollution governance.After two monthsof operation, the DO is maintained in the6mg/L, the degradation rate of COD, NH₃-Nand TP stable at38%,16%and27%, the river eliminate black and stink.PCR-TGGEtechnology and DNA extraction was applied to study microbial diversity for the waterbefore and after the repair,the result showed that microbial population abundance ofriver water after treatment has improved significantly. By forecasting, this technologywas applied to the whole river, the theoretical removal efficiency of COD, NH₃-N andTP were99%,83%,96%, the effluent can reach the five types of water, the waterreturned to normal.