Study On Composition And Antioxidant Activity Of Polysaccharides From Several Kinds Of Chinese Traditional Medicines

To study the separation and purification, physieoehemieal properties, structureanalysis and antioxidant activity of the polysaccharides from Cordceps militaris andPanax Ginseng in this investigation; to study the extraction and antioxidant activity ofthe polysaccharides from Orange peel and Astragalus in this investigation. The specificconclusions were obtained as follows:⑴F ourfractions of Cordceps militaris polysaccharide(CMP) and three fractions ofPanax Ginseng polysaccharides(GPS) were obtained by the technology of ultrafiltrationand graded ethanol precipitation.The higher activity CMP-1and GPS-1were furtherseparated by DEAE fast flow Sepharose anion-exchange chromatography to obtain fivefractions of CMP-1,seven fractions of GPS-1.The higher content CMP-1-1and GPS-1-1were further purified by Sephadex G-75gel-permeation column to receive the singlepolysaccharide.The chemical compositions of CMP-1-1including xylose (Xyl),mannose(Fru),glucose(Glu) and galactose(Gal) were determined by capillary zoneelectrophoresis. he chemical compositions of GPS-1-1including mannose(Man),glucose(Glu) and galactose(Gal) were determined by HPLC.⑵A ntioxidant activity tests revealed thatCMP and GPS showed good capability ofactivity of hydroxyl radical, superoxide anion radical and DPPH radical, and thescavenging ability increased with increasing polysaccharide concentration.GPS-1andCMP-1have the strongest activity among the other fractions.⑶T he microwave extraction of orange peel crude polysaccharides(OPP) optimalparameters as follows:extracting time18min,the ratio of solid and liquid1:25g/mL,microwave power250W.Under these conditions,the extraction yield of crude polysaccharide with ME was33.71%.The complex enzyme extraction of Astragaluscrude polysaccharides(APS) optimal parameters as follows:cellulas1.5%,pectic1.0%and papain0.5%, extracting time126min, extracting temperature52.5℃, pH6.Underthese conditions, the extraction yield of crude polysaccharide was4.81%. Antioxidantactivity tests revealed that OPP and APS showed good capability of activity of hydroxylradical, the scavenging ability increased with increasing polysaccharide concentration.