Studies On The Development And Efficacy Of Health Care Products Related To Ginseng And Astragalus

With the improvement of living standards, people more and more emphasis on the importance of keeping in good health, pay attention to their health and prevent the happening of the disease.As is known to all, the function of the immune system is closely related to health,so the health care products which can enhance immunity gradually goes into people’s vision.As tonic medicine,Ginseng and Astragalus are recommended by the famous doctor in the past dynasties since ancient times.Both of them have saponins and polysaccharide components,witch can enhance immunity,especially secondary saponins of Ginseng has more significant activity.In this paper,we prepared Shenqi chewable tablet,the function of this product and other health care products related to Ginseng and Astragalus witch were prepared by our group was evaluated,we got the following results:1. Exacting the saponin and polysaccharose from Ginseng and Astragalus with the method of Water-extraction and alcohol-precipitation and separating them.Extraction process was optimized by orthogonal experiment,in addition, the condition of saponin transformation was investigated.The best method was: adding 8 times amount of water and extracting the herbs three times, each for 1.5h.Concentrating the extracted fluid and filtering after alcohol-precipitation. Collecting on clear liquid and concentrating.The number 4 condition was the best transformation condition.2.The main components in the transformation sample were identified by liquid chromatography combined with mass spectrometry.The result showed that the sample mostly contained ginsenoside Rb1,Rd,Re,Rf,20S-Rg3,20R-Rg3, Astragaloside IV and so on.There were 14 kinds of components in all.The main secondary saponin was ginsenoside Rg3.3.The excipients were screeninged and we got the following molding process:The proportion of extract powder and excipients was 3:1.The excipients were composed of mannitol and sucrose.The corrigent was aspartame and citric acid.Putting them together and mixed thoroughly.Moistenning them with 85% ethanol and prilling them with 18 mesh.After being dried for 2h at 60℃,stabilizing the size of particles.Then adding the magnesium stearate into the particles to mix together and regulating the pressure of the upper punch and lower punch in the tablet press to make sure that the tablet-weight was about 0.5g.4.HPLC was used to determine the content of ginsenoside Rg3 in the Shenqi chewable tablet. The mobile phase was acetonitrile and waters,the detective wavelength was 203 nm.The result showed that the linear range was from 2.5 μg to 15 μg. The content of ginsenoside Rg3 in the Shenqi chewable tablet was 0.7mg/g.5.MTT test was used to study that how the Shenqi chewable tablet,Fufanghuangqi chewable tablet,4-day Shenqizhi ferment powder and 7-day Shenqizhi ferment powder influence the mice splenocyte proliferation.The result showed that the four health care products could significantly promote mice splenocyte proliferation with Con A induced and LPS induced.They had the effect of cellular immunity.6.The method blood coagulation was used to study humoral immunity of Shenqi chewable tablet,Fufanghuangqi chewable tablet, Shenqizhi ferment powder.The result showed that they could significantly improve the level of antibodies and spleen index.They had no influence on the weight of mice except that the low dose group of Shenqizhi ferment powder could significantly inhibit the growth of body weight of mice.So the three health care products had effect of humoral immunity.