Preparation, Analysis And Activity Evaluation Of Saponins In Yaotongning Prescription, Notoginseng And Ginseng

In this thesis, one hundred fifty-four prescriptions of Chinese Traditional Medicine (TCM), which were used to treat rheumatism, were researched according to the principle of constructing these prepractions of activating collaterals, dispelling cold, enriching blood and relieving pain, and the number of occurrence of four hundred and one herbs in these TCMs was statisticed. The herbs that appear mostly in the prescriptions were Radix Angelicae Sinensis (fifty-eight times), Radix Angelicae Pubescentis (forty times), Rhizoma Ligustici Chuanxiong(thirty-seven times), Rhizoma et Radix Notopterygii (thirty-six times), Radix Achyranthis Bidentatae (thirty-five times) and Radix Glycyrrhizae (thirty times), respectively. Based on the above results and the prescription of Yaotongning Capsule, four herbs, Radix Glycyrrhizae, Radix Notoginseng, Panax Ginseng C.A.Mey. and Radix Cyathulae, were selected as material herbs. The preparation technology and quality analysis of the saponins from the four herbs were studied. The activity of the saponins to inhibit xanthine oxidase in vitro was also investigated further.The extraction method coupling solvent reflux and macro-porous resin adsorption was applied to extract saponins from the four herbs. Depending on the content of total saponins determined by the vanillin-perchloric acid colorimetry, the opertation conditions for preparing the saponins were optimized. The content of total saponins of the extracts were74.87%(Radix Glycyrrhizae),84.75%(Radix Notoginseng),88.53%(Panax Ginseng C.A.Mey.), and15.74%(Radix Cyathulae), respectively. Furthermore, the determinations of ammonium glycyrrhizinate in glycyrrizae extract and notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1in notoginseng extract by High Performance Liquid Chromatography (HPLC) were established respectively. The results showed that the content of ammonium glycyrrhizinate in the glycyrrhizae extract was34.69%, and the contents of notoginsenoside R1, ginsenoside Rg1and ginsenoside Rb1in notoginseng extract were13.50%,58.73%and12.52%.A HPLC-DAD method was established for simultaneously determining five saponins (ammonium glycyrrhizinate, notoginsenoside R1, ginsenoside Rg1, Re and Rb1) in the mixture saponin sample of glycyrrhizae, notoginseng and ginseng, which could be used for the simultaneous determination of these saponins in proprietary Chinese medicines containing the three kinds of herbs.The purine metabolic experiment indicated that saponin extracts from Radix Glycyrrhizae, Radix Notoginseng, Panax Ginseng C.A.Mey. and Radix Cyathulae could inhibit the activity of xanthine oxidase in vitro. Notoginseng saponins exhibited the highest activity to inhibit xanthine oxidase, followed by ginseng saponins. Their maximum inhibition rates were higher than20%. After being added alkonoids and flavonoids, the activities of all of the saponin extracts for inhibiting xanthine oxidase decreased but their trend of the inhibition rate was similar to that of pure saponins.