Fabrication And Property Of N-hydroxymethyl Acrylamide Polymer Brush

Polymer brushes have been extensively studied over the past two decades and have attracted considerable interest for a wide variety of applications in nanotechnology, molecular biology, biosensors and biomedical sciences. Surface-initiated ATRP (SI-ATRP) has been shown to be one of the most efficient ways to producing polymer brushes exhibiting high surface grafting density and low polydispersity.In this work, Poly(n-hydroxymethyl acrylamide)(PHMAM)-grafted silicon and quartz surfaces were successfully prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) at room temperature in aqueous condition and protein asorption, signal amplification performance and application in catalysis of the polymer brush were studied. Specifically, the dissertation includes the following five parts:1. Poly(n-hydroxymethyl acrylamide)(PHMAM)-grafted silicon and quartz surfaces were successfully prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) with methanol/water mixtures (4:1,2:1,1:1,0:1; v:v) as solvents and CuCl/2,2-dipyridyl as a catalyst at room temperature. To obtain information on the polymer brush layer, we characterized its surface structure and properties by using atomic force microscope (AFM), fluorescence probes, X-ray photoelectron spectroscopy (XPS), and static contact angle measurement.2. The homogeneous and well hydrophilic n-hydroxymethyl acrylamide polymer brushes are obtained successfully at room temperature in aqueous condition. The contact angle of n-hydroxymethyl acrylamide polymer brush modified surface reaches to5degree and the polymerization degree of the polymer brush is53.3. The ability of these surfaces to suppress protein adsorption was assessed by investigating the adsorption of fibrinogen and HSA on these surfaces and the results indicate that PHMAM-modified surfaces can resist protein adsorption.4. Fluorescent amplification performance was estimated by reacting with fluorescent substances containing pyrene moieties and the result shows that PHAM-modified surfaces have the added advantage of providing3-D coatings with higher binding capacities (moles of molecules per unit area) than self-assembled monolayers (SAM).5. The polymer brush reacted with the palladiumcyclic catalyst with the hydroxyl group to generate the catalyst-functionalized polymer brush that could catalyze the Suzuki reaction and exhibit high catalytic activity.