Studies On Structure And Property Of Electrospun Silk-Hylauronic Acid Nanofibers

Electrospun nanofibrous scaffolds can mimic extracellular matrix (ECM)morphologically. It has larger specific surface area and certain porosity, providing bettercondition for cells to adhere and proliferate. Native macromolecular materials such assilk fibroin(SF), chitosan, gelatin, hylauronic acid(HA) et al has good biocompatibilityand biodegradability, which has been reached a lot home and aboard recent years.SF and HA were totally dissolved in formic acid, and then were electrospun intofibrous membranes successfully. SEM, FT-IR and XRD were used to observe themorphology of membranes and characterize the nanofibrous mats. Thermal andmechanical properties were also tested. The results showed that the average diameter ofnanofibers increased by increasing the concentration of solution, the molecular andcontents of HA. The condensed structure of silk fibroin in blend nanofibrous mats wasmainly SilkⅠ. The peak thermal degradable temperature and tensile strength increasedwith higher HA contents.1-ethyl-3-(3-dimethylaminopropyl) carbodiimidehydrochloride (EDC)/N-hydroxysuccinimide (NHS) were selected to crosslink theblend nanofiber. Compared with the uncross-linked nanofiberous membranes,EDC/NHS could promote stimulated SF to form Silk Ⅱ.Bone mesenchymal stem cells (BMSCs) were cultured on SF/HA nanofibrous mats.The pictures of H-E staining and SEM images showed that BMSCs could adhere andproliferate on the membranes. BMSCs maintained good shape on materials. And thegrowth of cells on SF/HA blend nanofibrous mats was better than pure SF nanofibers.These results showed that SF/HA nanofibers could support the growth of BMSCs andwere proved to be non-cytotoxic in vitro.