Effects Of Resveratrol On Redox State And Blood Pressure Regulation For Mice Fed With High-Fat Diet

This paper used resveratrol(RSV) intervening high-fat diet(HFD) mice, when obesityhad occured or not, to research the effects and possible mechanisms of different doses of RSVon the redox state, inflammation and blood pressure regulation, having positive practicalsignificances to prevent the occurrence of hypertension.4week-old Kunming mice were fed normal diet for one week, and then randomlydivided into4groups: normal diet, HFD(18%lard oil), HFD+0.015%RSV and HFD+0.03%RSV. After these mice given HFD for12weeks, they were divided into3types of obesityaccording to the body weight: obesity resistant, medium weight, obesity. Each category wererandomly divided into three dietary groups: HFD, HFD+0.03%RSV and HFD+0.06%RSV,and they were fed continuously for17weeks. Tail artery blood pressure was tested in the11thand28thweek, and samples were taked on the12thand29thweekend. The paper adoptedluminol chemistry shining method to test ROS lever, DTNB colorimetric method to testGSH-Px activity, spectrofluorimetry to test GSH/GSSG, kit methods to test activities ofT-AOC, CAT, NOS, ATPase and content of MDA, ELISA methods to test TNF-α, IL-6,MCP-1, ET-1, AngⅡ and renin contents, Cushman improving method to test ACE activity,and fluorescent quantitation RT-RCR method to analysis gene expression of p22phox, NFkB,p38, jun, AGT, ACE and AT1a in kidney.The experimental results showed that, adding resveratrol to intervene could be effectivein preventing the occurrence of oxidative stress, lowering levers of inflammatory markers likeTNF-α, IL-6and MCP-1, regulating levels of vasoconstriction factors like ACE, AngⅡ, ET-1andrenin, lowering blood pressure for the12-week HFD mice.0.03%and0.015%doses ofresveratrol both could significantly inhibite the inflammatory response for the mid-term HFDmice, and there was a dose-effect relationship.0.06%dose and0.03%dose of resveratrol both can significantly improve inflammatoryresponse of long-term HFD mice, but the0.06%dose had more markedly improving effectson the antioxidant capacity, RAS, vasoconstriction factors and blood pressure for long-termHFD mice. The higher the degree of obesity in mice, the lower the antioxidant capacity of theorgans and inflammatory responses, the higher levels of RAS components and thevasoconstriction factor, eventually leading to a rise in blood pressure.29-week HFD could inflation the mRNA expressions of p22phox, NFkB, jun and p38,and also could inflation the mRNA expressions of AGT, ACE and AT1a. Adding a goodamount of RSV could restain the abnormals of upper gene expression on the obesity, andthere were dose-effect relationships.There may be exist resistant mechanisms in theobesity-resistant mice on abnormal HFD-induced gene and physiological levels.