The Camellia Cake Extracts Improve Lipid Oxidation Stability Of The Role Of Research

In this experiment, the Camellia cake three extracts as testmaterials, evaluation of the antioxidant activity of extracts to the determinationof antioxidant indicators were measured in the extract on inhibiting lipidauto-oxidation, photosensitized oxidation, enzymatic oxidation,extract andBHT, BHA, TBHQ in inhibition of lipid oxidation synergy, extracts of totalphenolic content was determined by colorimetry, using high performance liquidchromatography for the determination of the components of polyphenols in thepolyphenol extract of Camellia cake and content. Camellia cake extract isadded to the grease inside the inhibition of lipid oxidation to lay the foundationto provide a theoretical basis for use for the the Camellia cake depthdevelopment. Specific research and research results are as follows:1. DPPH· radical, hydroxyl radical, superoxide anion scavenging effect ofdifferent types of camellia oil cake extracts （isopropanol extract, hexane extractand ether extract）, as well as the complexing ability of Fe²⁺and Fe3+reducingpower. The results showed that the isopropyl alcohol extract on DPPH radicaland hydroxyl radical scavenging ability and reducing power of Fe3+wassignificantly larger than that of n-hexane extract and ether extract. The hexane extracts of strong superoxide anion scavenging ability of the three extracts onthe complexation ability of Fe²⁺is not very significant difference. DescriptionCamellia cake extract has significant antioxidant activity, isopropanol extractantioxidant activity better.2.Use FRAP method to determine the three kinds of extract （isopropylalcohol extract, are hexane extracts and ethyl ether extract） total oxidationresistance, were3.29±0.12a,2.26±0.19b,0.89±0.15c, isopropyl alcoholextract always the strongest antioxidant capacity. Establish the oil to beautomatic oxidation model, the three extractions according to high and lowconcentration samples （0.5%,0.25%） are added to the does not add anyantioxidant corn oil, were determined, PV P-AV, TBARS and conjugateddienes （CD） value, it is concluded that the camellia cake extract has betterinhibition oil to be automatic oxidation of ability, ethyl ether extract inhibitoryability is the worst.3. Establish grease photosensitive oxidation models to peroxide value （PV）to determine index, continuous determination to join in camellia oil cakeextracts photosensitive oxidation of PV value system, and the resultsobtained can inhibit extract grease photosensitive oxidation of ability, isopropylalcohol extract the best effect is followed by cyclohexane extract. Use thestandard lipoxygenase, three extract （isopropyl alcohol extract, are hexaneextracts and ethyl ether extract） high and low concentration of lipoxygenaserespectively the inhibitory ability, the inhibition capacity for: IE high66.57%,58.46%lower NE IE, high54.64%, low40.58%, DE NE high14.90%,9.24% DE low.4.Establish isopropyl alcohol extract high and low concentration samplerespectively with the BHT, BHA, TBHQ three kind of common antioxidantinhibit oil synergy model, obtained the isopropyl alcohol extract and threekinds of antioxidants have synergy between. And then after the synergy ofspecimens, respectively for the wavelength scanning, concludes that the threekinds of antioxidants maximum absorption wavelength place still haveabsorbed wavelength, added of three commonly used antioxidant synergy arestill there after part.5.Through the three kinds of total polyphenols extract contentdetermination and using high performance liquid chromatography （HPLC）method to the analysis of the phenolic compounds are found, isopropyl alcoholextract, are hexane extract and ethyl ether extract of total phenols content inisopropyl alcohol extract the highest （9.23±0.33mg/g）, is followed bycyclohexane extract （6.45±0.61mg/g）, and ethyl ether extract the lowest（3.86±0.48mg/g）. The high performance liquid chromatographic detectionisopropyl alcohol extract contains table catechin, shaddock skin grain andcatechin, are hexane extract also contains table catechin, ethyl ether extract inthis way not to detect.