Construction And Immobilization Of Complex Bacteirum With High Efficient Phonel Degradation And Study On The Degradation Characteirstics

In the paper, four active and phenol-biodegradable bacterial strains were isolated from the activated sludge of the aeration tank of Jilin chemical wastewater treatment plant through screening and gradient-type domestication law(50mg/L～2000mg/L). The bacterial strains were named as WGN-1, WGN-2, WGN-3and WGN-4. Based on the preliminary study of bacterial growth and phenol degradation characteristics of WGN-1, WGN-2, WGN-3and WGN-4, and under specific environmental conditons that were100mL reaction system,30℃, pH=7.5,1000mg/L phenol concentration,1%total bacteria amount,125r/min shaker speed, the four bacterial strains were set throuth different combinatorial tests to find combination suit the most efficient and highest phenol degradation which had would be named as complex bacterium WGN.The experiment results showed that complex bacterium WGN, compared to the single bacterial strain, had better adaptation characteristics of various temperatures, pH values and substrate concentrations, and also higher phenol degradation. The optimal temperature inerval of phenol degradation for complex bacteriumWGN, strain WGN-1and strain WGN-4was30℃～35℃; The best temperature of phenol degradation for strain WGN-2was35℃, and for stain WGN-3that was25℃. The pH value interval of optimal phenol degradation for both the complex bacterium and single strains was7-8. For strain WGN-1and stain WGN-2, the optimal substrate concentration of phenol degradation is1000mg/L, and for train WGN-3and stain WGN-4that was500mg/L.Through the orthogonal experiment, the inspection of degradation efficiency, and the state observation of immobilized embedding balls, the optimal embedding conditon for the complex bacterium was polyvinyl alcoho, PVA (m/V):sodium alginate, ALG(m/V): CaCl2(m/V):bacterium amount(V/V)=9%:1.5%:3%:5%. Besides, the bacterium amount had the most effect on the phenol degradation efficiency of the embedding balls among all the four factors. SEM was used to describe the embedding balls of the complex bacterium. The phenol degradation efficiency of immobilized bacterium and free stated bacterium under different environmental conditons was also studied, and the results showed that immobilized bacterium performed better degradation efficiency and tolerance. The optimal degradation temperature interval for immobilized bacterium was30℃～35℃, and pH was7-8. The simple device for the preparation of immobilized balls was designed and made, and the phenol degradation of embedding immobilized embedding balls in the semi-successive reactor was imitated.