Synthesis Of Nanoparticles Assembly Based On DNA-nanoribbon And Its Application In Caner Detection

The principle of DNA specific binding can be achieved the design of DNAsequences, can reached the purpose of DNA nanostructures. DNA nano-materialshave been used in many ways, the use of DNA nano-materials as an componentbecome a hot topic in the biochemical analysis. Because of Nanopartical’s specialnature, it has been widely applied in various fields. For its special nature and itsgood biocompatibility of nanoparticles, it has been used in biochemical analysis.Quantum dot is one special kind of nanopartical, because of its fluorescencecharacteristics, so it can be used as imaging material. We has done the followingthree experiments:We complete and design one new type of DNA nano-ribbon. By useing maleicand sorbic acid cyclization, we prepared pecific sites on the DNA nano-ribbon. Useof the reaction, we functionalized of DNA nano-ribbon, and reached the target ofguid nanopartical assembly through DNA nano-ribbon. We use the fluorescencecharacterization of quantum dot reached the goal of characterization and propertiesof nano-ribbon.Then we used the DNA nano-ribbon, modified it with Ramos aptamer in orderto achieve the goal of DNA nano-ribbon binding Ramos cells. Through thefluorescence role of quantum dots, we can achieve a qualitative analysis and imageobservation of Ramos cell. Quantum dots can be solubled into metal ions through theacid, use DPASV we enrich the metal ions and detect the ions and quantum dots.Because the metal ions, quantum dots and Ramos cells have an indirectquantitativerelationship, we can qualitativly and quantitativly detect Ramos cell.In subsequent experiments we designed a simple method to detect specific short-chain DNA sequences. Through the use of enzyme tools, we achievedamplification of short DNA sequences, and then through the rolling circle reactionamplify DNA to an sequence of hybridization probes. The use of Au-HRP probecatalysis of the Luminol-H2O2chemiluminescence system, can achieve thequantitative detection of DNA sequence.