New Fluorescent Probes Based On BODIPY And9,10-phenanthroline Diamine

So far, Anion fluorescent dye to identify the research for a long time, but Anion Recognition in the aqueous phase which rarely reported.The probe1is a first phenanthroline quinone as a raw material, by hydroxylamine, and a chlorine acylated to give the intermediate K.9-anthracene-carboxylic acid as a raw material by reduction of chloride, a substituted reaction to give the intermediate fluorophore H. Finally, K and H in tetrahydrofuran as solvent, the reaction for five days in a nitrogen atmosphere to obtain the final product. The probes1the anion selectivity test showed that its phosphate ion and iodide ion in response to the fluorescence quenching of more than50%. More importantly, all anions tests are carried out under a solvent in the water phase, thus this probe design dye anion recognition in real biological environment has guiding significance.Also has certain guiding significance in the real biological environment design dye anion recognition in the future,although fluorescent molecular probes for the detection of thiol-containing alcohol-based amino acid has been some progress, the specific choice of cysteine probe research.still has a lot of problems. Strongly nucleophilic thiol group, we often by nucleophilic reaction to identification it and other amino acids. Nucleophilic cysteine thiol, the excellent performance of BODIPY (boron trifluoride diethyl pyrrole) as a chromophore, the quinoline ring chain BODIPY mother, synthesized a new class of BODIPY-based fluorescent probe2. Probe1,8-hydroxyquinoline as raw material, through hydroformylation chloride, amine-substituted and other steps to get. Strong nucleophilic reaction mechanism based on the mercapto group, the probe2can easily be substituted with a mercapto group by nucleophilic reaction, so that the fluorescence of the fluorophore in the intramolecular recovery successfully completed for the Cysteine high cysteine and glutathione. identification of three amino acids, and further by the type of the nucleophilic reaction rate, and can be open cysteine, homocysteine and glutathione distinction, such a reaction type OFF-ON fluorescent probe in the medical biological field has a very good application potential.