Separation And Enrichment Of Characteristic Components In Xinjiang Local Essential Oils Based On MIPs

Following the development of the essential oil industry in China, related nationalstandard have been formulated. But these standards are simply limited to the physicaland chemical factor of the oil, no detection methods were proposed to the lowconcentration components. Compared with European Union and the United States,some stringent standards have been performed, especially for the trace elementswhich have a larger impact on essential oil quality. To improve the essential oilquality of Xinjiang, it’s necessary to establish a rapid detect method for local essentialoil products. Molecular imprinting (MIP) technology is a novel type of separationtechnique with high efficient separation and molecular recognition. In this study, themonolithic MIP columns were prepared by combining with MIP and monolithiccolumn technology for separating and enriching the characteristic components of theoil.At first, ingredients of rosemary and pepper mint essential oil in Xinjiang wereanalyzed by gas chromatography-mass spectrometry, respectively. Two limitedcompositions of essential oil, camphor and menthofuran, were determined. And thentwo monolithic MIP columns were prepared focusing on these two components, andtheir performances were evaluated. Specific content as follows:(1) Components of the Xinjiang local rosemary and pepper mint essential oil wereanalyzed using GC-MS under optimized condition. The identified complexes werechecked with references, and two components were chose as characteristic componentfor two oil, camphor to rosemary and menthofuran to pepper mint essential oil,respectively.(2) Monolithic MIPs for camphor and menthofuran were prepared by in situpolymerization method as the liquid chromatography stationary phase. The MIPsshowed high affinity and selectivity for template molecules under the optimizing thepolymerization and chromatographic conditions. The column capacity, reactionprocess and kinetic model were determined by frontal chromatography,thermodynamic test, dynamic test, respectively.(3) The prepared monolithic column were used to enrich the characteristiccomponents camphor and methofuran from real essential oil of rosemary and peppermint, and the recovery rate, the detection limits and the quantification limits weredetermined.