Study On Genetic Diversity Of Culter Alburnus In The Yangtze River

Culter alburnus widely distributed in the eastern part of our country all river, isthe largest fish in eyprinida,Culterinae,it not only has a high nutritional value,economic value, also has a very high ecological value,it can maintain the stability ofthe ecological system waters. In recent years due to environmental pollution, habitatdestruction, water conservancy built, overfishing artificial influence, Culter alburnuswild resources dried up quickly, the population miniaturization and Low annualisedphenomenon particularly serious, to carry out Culter alburnus population protectionwork, at present there is no comprehensive system Culter alburnus geneticbackground research, hard for the work of protecting Culter alburnus provides thereliable theory basis. In this paper the determination of the Yangtze river system and agroup of Culter alburnus162tail control all the sequence of mitochondria, analyzesthe genetic diversity and population structure and population historical dynamic, forthe purpose of safeguarding the Yangtze river system Culter alburnus germplasmresources recovery and artificial proliferation discharge provides the theoretical guidebasis, specific results are as follows:1.Sequences of MtDNA D-loop gene of Culter alburnus were936bp long. Thetermination associated sequence domain, the central conserved domain and theconserved block domains were identified in the mtDNA control region of Culteralburnus, and the general characters of the functional units including ETAS, CSB-F,CSB-E, CSB-D, CSB1, CSB2and CSB3and so on were provided.In ETAS sequencecontains the core TGCAT and reverse complementary sequence ATGTA sequence,and in the termination associated sequence domain were found four core sequences ofTACAT. Poly(T) was found in the middle of control region, most of the sites are inthis variation poly (T) in front of the structure, and poly (T) structure of the seriesafter is relatively conservative a lot.2.The Yangtze river system, The haploid variety is0.866, nucleotide diversity is0.00330, present high single times type diversity and low nucleotide diversitycoexisting phenomenon that Culter alburnus of genetic diversity is low. Among them HeJiang is the highest mountain(Hd=0.879，Pi=0.00534), and YunYang isminimum(Hd=0.377，Pi=0.00132).3.The Yangtze river system, the14Culter alburnus groups are not form apparentlineage structure, no obvious geographic differentiation. Other groups in nanchonggroup and the existence of a highly genetic differentiation (Fst:0.50761~0.74012,Nm:0.17557~0.48501), YunYang group and tributaries of the three groups (innanchong, shiyan, jian river) also exist between high genetic differentiation (Fst=0.74012,0.31517and0.19235, Nm=0.175572,1.08645,2.09943), and other groupsexist only low differentiation between each other or have no obvious differentiation.Through the middle and lower reaches of the Yangtze river, the river and tributaries,mainstream and lakes, lake for AMOVA molecules and tributaries of varianceanalysis that, most of the differentiation exists in group internal, between each otheralmost no genetic differentiation.4.Through the Tajima ’s D and Fu’ Fs neutral testing and nucleotide not matchinganalysis, combining Culter alburnus high single times type diversity and lownucleotide diversity of coexistence characteristics, show that Culter alburnuspopulation may have undergone recent population expansion, expansion-time from10000to20000, as the late pleistocene.5. The lower reaches of Yangtze river group generally high genetic diversity, and thegenetic distance and genetic differentiation value are small, can be seen as a bigprotection management unit. The genetic diversity of the highest mountain group,second chizhou, need to be protected. Also with other groups in nanchong geneticdifferentiation between the largest, and YunYang groups of genetic diversityminimum, and because of its special geographical position, but through the specialway to the separate protection.