Correlative Enzymes Of Acremonium Hansfordii In Infection To Tetranychus Urticae Koch Had Synergistic Effect And Effect Of Infection Of Commom Acaricides To Its

Previous studied that Ahyl played a great role on infection to Cabbage caterpillarand AphidHost was infected by Ahyl, consumes its protein inside the body, destroy the protein synthesis of the tissues and organs, the body of detoxifying enzymes and protective enzyme lost balance.Tetranychus urticae Koch was used as experinmental acarids, studied on defense reaction of Acremonium hansfordii in Infection to Tetranychus urticae Koch and and effect of infection of Commom Acaricides to its.The results were as follows:1. The Ahyl of protease and chitinase activity were determination by Folin reagent and DNS method in different days. The Ahyl of amylase activity was determination by Hamilton method; the Correlation between amylase activity and mycelial dry weight were investigated.The period of Acremonium hansfordii in Infection to Tetranychus urticae Koch,the synergistic effect of hydrolyze enzymes were investigated.The result showed:There existed significantly difference (P>0.05) of protease treatment activity with different growing periods. The highest activities of protease treatment was0.3340.60U/mL in3days. There existed significantly difference (P>0.05) of chitinase treatment activity with different growing periods, treatment activity was always higher than control, the highest activities of chitinase treatment was220.86U/mL in4days.Before4days, Ahyl mainly mycelial growth and increment in the liquid culture, The highest mycelial biomass was occurred in4days, the highest amylase activity was occurred in5days.2. Detoxifying enzyme and protective enzyme activity were determination by in the infection process of Tetranychus urticae Koch under laboratory condition.The results showed that the activities of CarE,GSTs,MFOs,SOD,POD and CAT in infected Ahyl had changed in different degrees.The CarE activity was first decreased then increased in treatment, the highest activities of treatment CarE was4.9uM/mg.Pro/30min in4days. There existed difference (P>0.05) of GSTs activity between control and treatment in1to7days. The control of GSTs was significantly difference (P>0.05) in different days. The GSTs activity was first decreased then increased in treatment, the lowest activities of treatment was occurred in2days and the highest activities of treatment was4.24Δ OD·mg-1Pro·min-1in4days. There existed difference (P>0.05) of MFOs activity between control and treatment in2to5days. The treatment MFOs activity was on an increasing tendency in1to5days, the highest activities of treatment was2.91nmol·mg-1Pro·min-1in5days.The treatment was inoculated by Ahyl. After2days,the treatment POD activity was on an increasing tendency, The activity of treatment POD synchronization was always higher than that of the control. There existed an small difference of SOD activity in control and keep stable. There existed significantly difference (P>0.05) of SOD activity between control and treatment in2to3days.After3days, the activity of treatment SOD showed decreasing trend. The activity of treatment CAT appeared increasing trend that treatment was inoculated by Ahyl in2days.The highest activities of CAT treatment was0.334OD240/head/min in5days, there synchronization existed significantly difference (P>0.05) of SOD activity between control and treatment.3. The effect on conidial germination and mycelial growth of Acremonium hansfordii and synergistic effect was determined for pyridaben, chlorfenapyr, spirotetramat by spore germination and mycelium growth rate method, screened of a comparatively higher compatibility chemical acaricides with Acremonium hansfordii. The results showed that pyridaben,fenpropathrin, clofentezine, flufenoxuron, chlorfenapyr, spirotetramat, spirotetramat inhibited spore germination and mycelium growth under different concentration,the inhibition shown a increase in different degree. Pyridaben, chlorfenapyr, spirotetramat had a synergistic effect with Acremonium hansfordii against two-spotted spider mite. Pyridaben, chlorfenapyr, spirotetramat had an inhibiting action on the germination of conidial of Acremonium hansfordii and their inhibiting efficiencies were respectively27.67%、27.83%and29.22%at high concentration, their had an inhibiting action on the mycelial growth of Acremonium hansfordii and their inhibiting efficiencies were respectively20%、18%and18%,inhibition on some isolates by their was significantly reduced,their had a better compatibility with Acremonium hansfordii. The result of abioassay showed that it was obvious of the synergism against two-spotted spider mite when Acremonium hans/brdii(concentration of1×106spores/mL) mixed with pyridaben(25ug/mL), chlorfenapyr(0.83ug/mL), spirotetramat(60ug/mL) than single used Acremonium hansfordii after11days, three kinds of acaricides mixed with Acremonium hansfordii(concentration of1×106spores/mL) o f half lethal time were respectively5.38d,5.41d,and5.79d,with LT504.19d,4.16d, and3.78d respectively earlier than that of used Acremonium hansfordii (concentration of1X106spores/mL) alone Ahyl (concentration of1×106spores/mL) mixed with (1:5and1:10)Pyridaben(25[ug/mL), Chlorfenapyr(0.83ug/mL), Spirotetramat(60ug/mL) of half lethal time were shorten.