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The Genetic Effect Evalution Of Released Parent Hypophthalmichthys Molitrix

Posted on:2013-12-04Degree:MasterType:Thesis
Country:ChinaCandidate:X F LiFull Text:PDF
GTID:2233330371472015Subject:Zoology
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3-. Genetic diversity analyses by SSR and mitochondrial DNA(1) Genetic diversity analysised by SSR in2010and2011. The results showed the gentic polymorphism in these population was high. The three SSR mulitiplex-PCRs was accorded with paternity test standards.(2)17haplotypes were found in29specimens collected from XiangJiang foundation seed fish farm in2010by mitochondrial DNA. Haplotype diversity was0.9557±0.0189and nucleotide diversity was0.202416±0.100982.(3)14haplotypes were found in22Shishou section released siver carps in2010by mitochondrial DNA. Haplotype diversity was0.9481±0.0287and nucleotide diversity was0.058185±-0.030804.7haplotypes were found in23Jianli section released siver carp in2010. Haplotype diversity was0.9170±0.0344and nucleotide diversity was0.087984±0.045471.7haplotypes were found in41cultivate specimens which sampled frome Jianli section in2010. Haplotype diversity was0.8707±0.0383and nucleotide diversity was0.041036±0.021789.(4)12haplotypes found in27Shishou section released siver carp in2011by mitochondrial DNA. Haplotype diversity was0.8832±0.0398and nucleotide diversity was0.084086±0.043236.14haplotypes found were in20Jianli section released siver carp in2011. Haplotype diversity was0.9368±0.0392and nucleotide diversity was0.173238±0.088230.23haplotypes were found in32cultivate specimens which sampled frome Jianli section in2011. Haplotype diversity was0.9758±0.0147and nucleotide diversity was0.190632±0.094886.(5) The analysis of molecular variance (AMOVA) showed that the variation was from the within population, FS7=0.03127also indicated that the genetic variation was from within the population mainly.
Keywords/Search Tags:Parent sliver carp, Release, Genetic effect evaluation, Genetic diversityanalysis
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