The Fermentation Of Spent Mushroom Substrates On Soil-borne Diseases Of Cucumber Control And Influence On Soil Microbial Communties

In this study, a Trichoderma viride were isolated from the spent mushroom substrates(SMS),it has strong inhibitory effect of the two pathogens that were Rhizoctonia solani and Fusarium oxysporum. Through the plate confrontation experiment and found that the inhibition rates wer e as high as66.8%and87.5%.Order to obtain the best conditions for Trichoderma viride use the SMS from fermentation, through the design of an orthogonal experiment, the results show, under the inoculation level of15%, temperature of26degrees Celsius,pH value of5.5, water and SMS from the ratio of2to1and kept the fermentation time of7days, Trichoderma viride spore concentration have reached the highest as2.87×109/g.The SMS with the advantage of the growth of Trichoderma viride and the great number of unsterilizated SMS mixed and kept compost management, according to a certain percentage of urea supplement of nitrogen, control of water, generally in summer or winter fermentated1or3weeks, the spores of the Trichoderma viride can reach the level of utilization. The fermentation of SMS by Trichoderma viride from Cucumber damping-off and Fusarium Wilt, fermentatio n waste materials from the mixed soil ratio of10%and mix of soil time of the6days for the control effect of Cucumber damping-off and Fusarium Wilt, respectively reached85.6%and74.3%.Some important enzymes in cucumber studies have shown that fermentation of SMS by Trichoderma viride can significantly enhance the PPO, SOD, TTC, POD and PAL activity, as far as we know, these enzymes have something to do with the plant defence, so enhance disease resistance in cucumber.On soil microbial community survey, it can be found that fermentation of SMS by Trichode rma viride to promote the growth and colonization of bacteria and actinomycetes, thereby improvi ng the soil micro-environment of the potential positive effect on plant growth.