Study On The Optimization Of NSP Enzymes Combination In Vitro And Its Effection On Content Of Nitrogen Containing Compounds In Manure Of Broilers

The study was to investigate the effect of non-starch polysaccharides(NSP) enzymes on in vitrodigestive energy(IVDE) of broiler diets by using a two step method with pepsin-pancreatin,then usedquadratic regress-orthogonal rotary design to screen the optimum enzyme combination,then toinvestigate the effects of enzyme combination on production performance and content of nitrogencontaining compounds of broilers to determine the feasibility of enzyme combination.The first experiment was conducted to discuss the effects of NSP enzymes on the in vitro digestiveenergy(IVDE) of broiler diets of different content of NSP. The first step investigated the dose-responseof the NSP enzymes on the IVDE in diets using one-way randomized experimental design. Five NSPenzymes-xylanase, β-glucanase, cellulose, pectinase, β-mannanase were added to corn-soybean dietand wheat-soybean diet, from0to900μg/g. The second step screened the optimum enzymecombination using quadratic regress-orthogonal rotary design. The method of test IVDE is two-stage invitro digestion assay. The IVDE of broiler diet regulating by NSP enzyme appeared “S” state diagram.The strength of NSP enzyme manipulation of IVDE in corn-soybean diet is:xylanase> β-mannanase>β-glucanase> cellulose> pectase,in wheat-soybean diet the sequence is: xylanase> pectase>β-glucanase> cellulose> β-mannanase. Xylanase had the most strong effect on IVDE on bothcorn-soybean and wheat-soybean diets, improved0.24MJ/kg,0.40MJ/kg, respectively. Incorn-soybean diet the optimum enzyme combination was xylanase34836U/kg, β-glucanase6762U/kg,cellulose1159U/kg, pectase873U/kg, β-mannanase24536U/kg; and in wheat-soybean diet theoptimum enzyme combination was xylanase65406U/kg, β-glucanase10132U/kg, cellulose980U/kg,pectase502U/kg, β-mannanase5141U/kg.The second experiment was to study the effect of non-starch polysaccharides enzyme onperformance, uric acid, urea and ammonia in manure of broilers.208one-day old Arbor Acres malebroilers were randomly divided into four groups(corn-soybean diet with NSP enzymegroup,corn-soybean diet group,wheat-soybean diet with NSP enzyme group,wheat-soybean dietgroup),each with4replicates. The composition and level of NSP enzyme in corn-soybean diet andwheat-soybean diet were from the first experiment. The results showed:, wheat-soybean diet group hadlower daily weight gain than wheat-soybean diet+E group during1-21days (P<0.05); wheat-soybeandiet+E group had lower F/G than wheat-soybean diet group during1-21(P<0.01)、21-42(P<0.05)、1-42(P<0.01) days respectively; the F/G of corn-soybean diet+E group was lower than corn-soybeandiet group by6.6%、4.7%、5.3%during1-21、21-42、1-42days,respectively (P>0.05). The manureammonia concentration of corn-soybean diet+E group and corn-soybean diet group were significantlyhigher than wheat-soybean diet+E group and wheat-soybean diet group (P<0.01) from2to6week age;the ammonia concentration of corn-soybean diet+E group was significantly higher than wheat-soybean diet group (P<0.01) at3week age. Compared with wheat-soybean diet+E group and wheat-soybean dietgroup, the uric acid content of corn-soybean diet+E group and corn-soybean diet group weresignificantly improved (P<0.01) in3to6week age. Compared with corn-soybean diet group, the ureacontent of corn-soybean diet+E group were significantly improved at2(P<0.05) and5(P<0.01) weekage,respectively.