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The Effect Of Additives On The Quality Of Hybrid Pennisetum Purpureum Silage

Posted on:2013-06-01Degree:MasterType:Thesis
Country:ChinaCandidate:Q F HuangFull Text:PDF
GTID:2233330374462913Subject:Animal Nutrition and Feed Science
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The object of research was concentrated on hybrid penisetum silage under different additiveswith different concentration,as well as studied the use of the Cornell net carbohydrate and proteinsystem net (CNCPS) to assess the nutritional value of silage, The purpose of study would like toprovide directions for the exploitation of hybrid penisetum purpureum silage.The results showedthat:Experiment one: In order to research the effect on hybrid penisetum purpureum silagequality by using the cornstarch or bentonite.Corn flour and bentonite were chosen as testmaterials.and a hybrid pennisetum purpureum height of2.5M was used as the silage material.TheCorn flour or bentonite levels were0%,3%,6%,9%respectively. Ensilaged for forty days atnormal temperature, the pH value, dry matter recovery (DMR), ammonia nitrogen (NH3-N),non-protein nitrogen (NPN) and other indicators were measured with three replicates for eachtreatment. The results indicated: Corn flour treatment improved silage fermentation quality, andhad significant effect on NH3-N/TN,DMR、the content of CP, NDF and ADF(P<0.01).The pHvalues with the increase in the amount of corn flour continued to lower in which ADF and NDFcontent decreased with up to30%, the content of LA, DM and Starch increased linearly.Theanalysis of Protein and carbohydrate composition showed that of9%level the nutritional valuewas best: The PC/CP and PB3/CP was higher, The CA/CHO and CB1/CHO proportion werehighest.(2) Moderate and high level content of bentonite improved silage quality that couldsignificantly improve DMR and the content of LA,CP, PB1in silage, also lower pH values,NH3-N/TN,GLR and PB3content to increase the feeding value indirectly. Considerd with sensoryidentification and CNCPS indicators, it was that the6%level of bentonite had the highestnutritional value.Experiment two: In order to research the effect on hybrid pennisetum purpureum silage qualityby using fermented green juices which was chosen as test materials.and a hybrid pennisetumpurpureum height of2.5M was used as the silage material.Ensilaged for forty days at normaltemperature, the pH value, dry matter recovery (DMR), ammonia nitrogen (NH3-N), non-proteinnitrogen (NPN) and other indicators were measured with three replicates for each treatment. Theresults indicated: Different concentrations of green juice fermentation could significantly improvethe quality of silage that had a significant increase in the content of lactic acid, CP and SP, asignificant reduction in pH. The green juice fermentation also could make better the silage proteinand carbohydrate composition: Treated silages’ PC content was significantly (P <0.01) reduced,PB1, PB2, and CA was significantly (P <0.01) increasedExperiment three: In order to research the effect on hybrid pennisetum silage quality byusing Enzymes which was chosen as test materials.and a hybrid pennisetum purpureum height of2.5M was used as the silage material.Ensilaged for forty days at normal temperature, the pH value,dry matter recovery (DMR), ammonia nitrogen (NH3-N), non-protein nitrogen (NPN) and other indicators were measured with three replicates for each treatment. The results indicated: Addingenzyme to the hybrid pennisetum purpureum got a good silage quality that the lactic acid contentof all processed was more than80%. Treatment groups significantly reduced silage fiber content(P <0.01), and increased Crude protein content by27.59%、45.47%、45.47%、60.38%、84.33%.If the lactic acid content was the main basis for judging silage effect, combined with the CNCPSindicators could be drawn the overall result:300mg·kg-1 xylanase+300mg·kg-1 cellulase>600mg·kg-1 cellulose>300mg·kg-1 cellulose>600mg·kg-1 xylanase>300mg·kg-1 xylanase.Experiment four: In order to research the effect on hybrid pennisetum purpureum silagequality by using Lactic acid bacteria and enzyme mixture. Acid bacteria and enzyme mixture werechosen as test materials.and a hybrid pennisetum purpureum height of2.5M was used as thesilage material.Ensilaged for forty days at normal temperature, the pH value, dry matter recovery(DMR), ammonia nitrogen (NH3-N), non-protein nitrogen (NPN) and other indicators weremeasured with three replicates for each treatment. The results indicated: Low levels of lactic acidbacteria (2.5g/kg) or moderate level of enzyme mixture (1.3g/kg) bacteria significantly reducedsilage’ pH, GLR, and ADL content, also significantly improved DMR and the content of DM, CP,and lactate, But the later was also better to improve the silage protein and carbohydratecomposition: CA/CHO and CB1/CHO was higher, the CC content significantly was reduced, thePA/CP, PC/CP and PB3/CP were both the low proportion with higher proportion of PB2/CP. Theenzyme mixture (1.3g/kg) could improve the fermentation quality, the decline in pH valuereduced rapidly with the increase in the amount of the LA bacteria in which the lactic acid contentwas synchronized changed and the ADF content with a significant reduction did not changesignificantly between the treatments. When enzyme mixture was1.3g/kg or1.8g/kg, lactic acidbacteria added did not improve carbohydrate and protein components that the content of CA, CB1and PB2did not significantly increase. In the low-level group of Enzyme mixture with the increasein the concentration of lactic acid bacteria, the nutritional quality was the best, when enzymemixture and lactic acid bacteria were respectively0.8g/kg and7.5g/kg.Its PB1content was thehighest with low PB2, CC and PB3content in which CA/CHO and CB1/CHO were the highest...
Keywords/Search Tags:Hybrid penisetum purpureum, silage, additives, nutritional value
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