Study On Extract, Determination And Activity Of Antioxidant Compounds From Date Honey

Honey is nature sweet substance produced by Aips cerana Febricius or Aipsmellifera Linnaens honey bees from nectar or secretions of blossoms, which honeybees collect, transform, store and leave in the honey comb, with high nutritional value.Honey are effective in reducing the risk occurrence of the heart disease, cancer,cataracts, different inflammatory processes and inmmune-system decline. Date honeyis one of bee honeys in china, its extraction against superoxide anion radical, hydroxylradical, lipid oxidation of free radicals and human serum lipoprotein oxidativemodification. In this paper, extraction, compositions, content of antioxdantcomponents from date honey are determined. The main contents include the followingseveral aspects.1. Date Honey dissolution conditions: Extraction ratio and DPPH radicalscavenging activity of antioxdant components from date honey were compared indifferent dissolved conditions of ultra-pure water or acidic water. The results showedthe extraction ratio of antioxdant compounds is9.0±0.2mg/10g honey, and theDPPH radical-scavenging activity is60.3±0.1%in the PH=2acidic water dissolvedconditions.2. Extraction column material selection of antioxidant components from dateHoney: The results of static adsorbtion of AB-8, XAD-2, D101, Polyamide resinshowed adsorbtion and desorption ratio of the antioxidant components from datehoney were76.7%,97.1%respectively by used XAD-2resin. And desorption againstthe DPPH radical scavenging was51.9±0.5%, significantly better than the otherthree resins. Therefore, XDA-2resin as the best column material.3. Optimization of dynamic adsorption and desorption conditions by usedXAD-2resin: the effect of honey/resin ratio, absorbtion flow rate on absorbtion, andthe effect of elute concentration, desorption flow rate, elute volume on desorption.Through comparing DPPH radical-scavenging activity of antioxidant compoundsfrom desorption, the optimum conditions of absorbtion and desorption by usedXAD-2resin were determined that honey/resin（1:1）, absorbtion flow rate（1.0 mL/min）, elute concentration（80%enthanol）, desorption flow rate（4.0mL/min）, elutevolume（2BV）.4. Study of antioxidant activity of the active components from date Honey:Bioactive components from Date honey against DPPH radical scavenging wereanalysed with HPLC-DPPH, activity of known bioactive components against DPPHradical scavenging were investigated. The results showed that twelve kinds ofbioactive components against DPPH radical scavenging were found in Date honey.Four kinds of known bioactive components were determined in bioactive components—caffeic acid, p-coumaric acid, naringenin, and kaempferol. Significant differencewere found in their scavenging capacity, kaempferol IC₅059.01±0.41μmol/L, caffeicacid IC₅069.37±0.33μmol/L, and p-coumaric acid, naringenin against DPPHscavenging were weak, which closely linked to molecular structure of bioactivecomponents.5. The content and antioxidant activity of the five known compositions from DateHoney. The results of HPLC showed the highest content of chrysin, followed bynaringenin, content of p-coumaric acid and kaempferol is quite, caffeic acid was thelowest. The DPPH radical scavenging activity evaluation results showed that chrysinnot against DPPH radical scavenging activity, the scavenging ability of the other fourcomponents are different.6. Enrichment, purification and preparation of unknown antioxidant componentsfrom date Honey: antioxdant components from date honey by acetone extraction, theextraction ratio is3.6±0.5mg/10g honey, and the DPPH radical-scavenging activity is94.7±0.1%, its main antioxdant component is1#absorption peak of HPLC-DPPHmethod, the peak area is49.2%.Though purified by LH-20and analyzed by HPLC,the result showed that LH-20had a good purification effect of antioxdant componentsfrom date honey, and obtained the1#absorption peak, which the peak area up to89.4%.