Tissue Culture System Of Malus Halliana

The Malus halliana originating in the Southwest, South, East of China, belong to Rosaceae Malus, also known as Begonia flowers, weeping crabapple, Jie Yuhua and so on.It is deciduous tree,flowering from March to April, the flower is very beautiful when flowering time, people love the Malus halliana very much, the ancients called it is the "flower in the fairy", its branches Bending after wind erosion or old is also a interesting sight.It’s Robust and easy to grow, so widely used in landscaping.The Malus halliana also have some edible and medicinal functions besides its garden ornamental value, so causing a great deal of attention of the relevant industry.How to breeding a large number of Malus halliana quality seedlings quickly to meet the market demand become a key. Now, the domestic Studies on Malus halliana were concentrated in cultivation, cuttings, grafting were more used for breeding, low survival rate and small propagation coefficient, can not meet the production needs, Severely limits the development of of Malus halliana,.tissue culture can improve its grow speed and reproduction rate. Malus halliana is a woody floral, its tissue culture is difficult, now few study on the Malus halliana tissue culture.Therefore, This experiment study from explants disinfection establish the sterile system to Rapid propagation in vitro conditions, induction of callus and rooting and acclimation of transplanted, Series of studies provide a systematic basis for Malus halliana tissue culture techniques. Specific results are as follows:1.Explant disinfection:the young explants Collected April to August each year have the best disinfection and sterilization, Sanitizing effect from good to bad is:young stem segments of single bud>young leaves>petal, the best disinfection treatment for stem segments is:75%alcohol30s+0.1%HgCl26min, the best disinfection treatment for leaf is:75%alcohol30s+0.1%HgCl28min, the best disinfection treatment for peta is:75%alcohol30s+0.1%HgCl26min.2.Rapid propagation in vitro culture:the Semi-lignified stem segments of Malus halliana which have the length of1.0-1.5cm have the highest sprouting rate:80%,orthogonal test to screen out the best concentration of medium and Hormone for Malus halliana bud induction start is: WPM+6-BA1.0mg/L+NAA0.5mg/L,start rate of86.67%, the best medium and hormone concentration ratio for shoot proliferation subculture is:MS+6-BA1.5mg/L+IBA0.5mg/L,1.0g /LAC,1mg/L of GA3help promote bud proliferation and elongation.3.Callus culture:the way of stem segment-line or supine, leaf adaxial face up can improve callus induction rate, Dark culture produce callus more quickly and the callus are better,30g/Lis best for produce callus, the best medium and hormone concentration for Callus induction is: MS+6-BA0.5mg/L+2,4-D0.5mg/L, callus induction rate is95%, NAA was better than2,4-D as auxin for callus proliferation,the best medium and hormone concentration for Callus proliferation is:MS+6-BA2.0mg/L+NAA0.6mg/L, the best medium and hormone concentration for Callus differentiation is:MS+6-BA2.0mg/L+NAA0.8mg/L, differentiation rate is88.33%.4.Rooting:the best medium and hormone concentration for rooting is:1/2MS+IBA0.5mg/L,the average number of roots was5.78, rooting rate was72.67%, and the root were thick with no callus, the best activated carbon concentration is1.0g/L, too high concentration is bad for rooting.5. Hardening and transplanting:tissue culture transplanting will be influenced by many factors, The results show:the way that place the shoots in natural light3d with the bottle is not open and Indoors with the bottle is open is best, use the glass beaker to cover transplanting seedlings will produce high transplant survival rate of80%, the best transplanting period for Malus halliana is April each year, the transplant survival rate is85%, the best culture medium is Vermiculite:peat: sand (2:3:1), anb its transplant survival rate is90%, the shade treatment of60%light transmission rate had higherr survival rate and the plant grow better.