Investigation On Rapid Propagation Of Multipurpose Woody Plant Moringa Oleifera Lam

In this paper, the establishment of Moringa tissure culture system and related mechanismwere studied. Establish the Moringa’s callus regeneration system by the mean of organogenesis.The main results were as follows:1. Fresh seeds were more responsive for developing plantlets. To disinfect the freshpeeled M. Oleifera seeds with1‰mercuric chloride for10mins, after cultured for10days, theseed’s germination rate was77.92%, and the plantlets were robust. In contrast, the contaminationrate of the one-year-stored seeds raised by once and the germination rate reduced by nearly8times against fresh seeds, and the plantlets were thin. When the shoot was sterilized with1‰mercuric chloride for10mins, the success rate was low only11.07%.2. In this study, three types of callus which could subculture were achieved, namedrespectively as type-Ⅰ, type-Ⅱ, and type-Ⅲ. The type-Ⅰcallus was yellow and the surface wascovered with glutinous substances, type-Ⅱ callus was white and tighten, the type-Ⅲcallus wasyellow and grainy. The optimal medium formula for type-Ⅰcallus was MS+2.0mg/L2,4-D+1mg/L KT, for type-Ⅱ callus was MS+1mg/L2,4-D+0.5mg/L KT, for type-Ⅲ callus wasMS+0.5mg/L2,4-D. The callus’s form related to growth cycle as well as physiological andbiochemical character in itself. Callus in the period of proliferation had stronger activity thanother periods, which was appropriated for the differentiation. Among three types, the type-Ⅲ hadthe strongest activity.3. To establish the Moringa rapid propagation system with the plantlet’s hypocotyl by themean of organogenesis. It found that the best medium for plantlet’s hypocotyl to induce bud wasMS+2.0mg/L6-BA. The addition of6-BA could range from0.5mg/L to0.8mg/L, NAA rangefrom0.2mg/L to0.3mg/L could make the proliferation rate more than7times.4. To establish the Moringa rapid propagation system with the shoot by the mean oforganogenesis. MS basic medium was the best medium for shoot to induce buds. The type andconcentration of exogenous hormones had profound effects on bud’s multiplication. The bestmedium for bud’s multiplication was MS+0.8mg/L6-BA+0.2mg/L NAA, proliferation rate was4.3times.5. In terms of the maximum percentage of rooting was obtained on1/2MS, the best medium for rooting was MS+0.5mg/L IAA, the rooting rate was78.06%, and the seedlingswere in good condition as well as roots. The optimal transplanting substratum was mixed perlitewith nutritional soil, after sterilizing, the substratum was good in permeability and moisture. Thisculture medium could meet the demand of water without roots rotted. The rooted plantlets weresuccessfully established in the soil, the survival rate was22.50%.