Study On Detection Of Seed Purity Of Two-line Hybrid Rice By Simple Sequence Repeat (SSR)

In this study,With the seeds of two-line hybrid rice varieties Liangyoul7, Benliangyou639, Tianliangyou616as the materials, we screen out several candidates from44pairs of primers. In our results, the same SSR primer was identified from one variety with two different methods. Primers identified from two-line hybrid rice can be used in detection purity of the seeds in laboratory without parents.In this research, we classify large and small seedlings according to <Rules for agricultural seed testing> after germination. We detect seeds purity of classified seedlings in laboratory with the method of SSR. In our result, the size of seedlings has a major effect on results, with a difference of5.3%. The small seedlings have no effects on results because being lethal in the farm. In conclusion, sampling methods is a key factor to the different results of detection of seeds purity in laboratory or farm.In this study,During detection F1groups seeds purity of two-line hybrid rice variety Liangyoupei9, we adulterate another different variety Xinliangyou6380F1group, collect leaves one by one in field, and detect adulterated Xinliangyou6380with the method of SSR exactly. We observe the same lane between Xinliangyou6380and Liangyoupei9sterile line during detection in the laboratoryIn this study,According to primer sorting results, we choose nine two-line hybride seeds, all of which can be detected by the SSR with RM17primer, and then, we can identify molecular differences in different varieties. In the same time, we discuss discretion and dependability of SSR method according as morphological and biological differences in the field. In our result, we prove that one pair of primer can’t differentiate the varieties with similar genetic background.In this study, we discovery that different results come up with different detection primer, during we detect one sample purity with different primers. The error from purity detection results in SSR method with different primers reduce gradually when filed sample purity increasing. The detection results in the laboratory might be higher or lower than those in the field in different varieties, so we can’t got a system error correction method to suit purity detection both in the laboratory and in the field.In this study, we accomplish the detection alignment between results of filed and laboratory and we discovery that different methods have different estimation abilities. We analyze the reason why one plant have different detection results. In the end, we claim several technical policy aiming to reduce errors in molecular detection and results from filed.