Studies On Fermentation Quality Of Alfalfa Silages At Different Temperature

There are four experiments in this study. This study was carried out to evaluate the effects of different temperature, previous fermented juice, cellulase and previous fermented juice+cellulase, on the fermentation quality of alfalfa silages during ensiling.Experiment I:Fermentation quality of alfalfa silages at30℃.Experiment I was carried out to evaluate fermentation quality of alfalfa silage at30℃.The silos were opened on1,3,5,7,14, and28th day of ensiling, the fermentation quality was determined. The DM content had not greate change during ensiling. The pH value decreased rapidly (p<0.05) in the first3days, and then the change of pH value was small after5th day of ensiling. The lactic acid content was not detected on the first day and the lactic acid content had a significant increase (34.12g kg-1DM). After7days of ensiling, the changes had not greate change. The butyric acid content continued to increase after the first day of ensiling. Total VFAs content reached to max (25.55g kg-1DM) on the28th day. The ammonia/total ammonia increased during ensiling, and showed the highest content (83.21g kg-1TN) on the28th day. In the end of experiment, the alfalfa silage had high pH value (5.15) and low lactic acid (50.62gkg-1DM) content, it was suggested that the fermentation quality of alfalfa silage at30℃was poor.Experiment II:Fermentation quality of alfalfa silages at40℃.Experiment II was carried out to evaluate fermentation quality of alfalfa silage at40℃. The silos were opened on1,3,5,7,14, and28th day of ensiling, the fermentation quality was determined. The DM content had not greate change during ensiling. The pH value continued to decline during ensiling, and showed lowest pH value (5.08) on the28th day of ensiling. The lactic acid content continued to increase and reached to the max (51.67g kg-1DM) on the28th day of ensiling. The butyric acid content continued to increase after the first day of ensiling, and reached to the max (0.56g kg-1DM) on the28th day. Total VFAs content reached to max (24.46g kg-1DM) on the28th day. The ammonia/total ammonia increased slowly (p>0.05) after3days of ensiling and increase significantly (p<0.05) on the7th day, and reached to the max (69.22g kg-1TN)(p<0.05) in the end of experiment. The alfalfa silage had high pH value (5.15) and low lactic acid (50.62gkg-1DM) content, it was suggested that the fermentation quality of alfalfa silage at40℃was poor.When compared with fermentation quality of alfalfa silages at30℃, the pH value of alfalfa decreased more rapidly, an also it could not make well fermentation quality at40℃Experiment III:The effects of additives on fermentation quality of alfalfa silages at30℃This experiment was carried out to evaluate the effects of adding previous fermented juice, cellulase and previous fermented juice+cellulase on fermentation quality of alfalfa silages at30℃during ensiling. All treatments were sampled after1,3,5,7,14, and28th day of ensiling. The DM content had not greate change during ensiling. The pH value showed rapid decrease in the initial stage of ensiling; the lactic acid content increased significantly (p<0.05) after5th days of ensiling, and the pH value of all additives treatments was lower (p<0.05) than the control. The butyric acid increased slowly during ensiling. The ammonia/total ammonia of all additives treatments increased slowly after7th day of ensiling. The ammonia/total ammonia of all the additives treatments was lower than the control (p<0.05).The lactic acid of all additives treatments were higher than the control (p<0.05). It indicated that all additives treatments improved the fermentation quality and feed value of alfalfa silages at30℃as compared with control; however PFJ+CF treatment is best.Experiment IV:The effects of additives on fermentation quality of alfalfa silages at40℃This experiment was carried out to evaluate the effects of adding previous fermented juice, cellulase and previous fermented juice+cellulase on fermentation quality of alfalfa silages at30℃during ensiling. All treatments were sampled after1,3,5,7,14, and28th day of ensiling. The DM content had not great change during ensiling. The pH value showed rapid decrease in the initial3day of ensiling. The pH value showed rapid (p<0.05) decrease in the initial stage of ensiling; the pH value of all additives treatments was lower (p<0.05) than the control.the lactic acid content increased significantly (p<0.05), the lactic acid content of all additives treatments were higher than control (p<0.05). The content of butyric acid increased slowly during ensiling. The ammonia/total ammonia of all additives treatments increased slowly after stored7days. The ammonia/total ammonia of all additives treatments was lower than the control (p<0.05). It indicated that additives treatments could improve the fermentation quality of alfalfa silages at40℃, and also feed value of alfalfa silages was improved. PFJ+CF treatment effect is best.