Non-Heading Chinese Cabbage Oguracms Combining Ability And Biochemical Characteristics Analysis And Issr Marker Of Male Gene

Non-heading Chinese cabbage (Brassica campestris ssp.chienesis Makino) was Common name cabbage, vegetables, natived to China, the main producing areas in the Yangtze River areas, the annual supply of vegetables, played an important role. Chinese cabbage was a typical cross-pollination of crops, heterosis obvious advantages. Chinese cabbage male sterile line was not out of self-incompatibility, and the shortcomings of the Department of Seed, to became a Chinese cabbage production of the ideal of a generation hybrid system, therefore, male sterility in research and application by the breeders of all ages. Non-heading Chinese cabbage OguraCMS was one main object of not male-sterile Chinese cabbage, Ogura male sterile source was completely male sterile sterility and infertility rates were100%of plants that had attracted much attention, while Ogura sterile transfer to anther crops initially for a long time, there was sterile plants turn yellow seedling stage, nectary dysplasia problems. Cruciferous vegetables, Jiangsu Academy of Agricultural Sciences research group from the Suzhou city, the introduction of vegetables Ogura sterility source, the success of their transfer to non-heading Chinese cabbage, after a multi-generational selection work, now had the non-heading Chinese cabbage OguraCMS largely overcomed plant seedling yellowing, nectary dysplasia problems. In this study, Non-heading Chinese cabbage OguraCMS combining ability and physiological characteristics of two aspects of research and analysis for further wide application in breeding and provide a theoretical basis, in addition a non-sterile gene ISSR molecular markers, not only to develop a mark, but also expected to explore and provide a theoretical mechanism of infertility about OguraCMS. The results are as follows:1、Combining ability and heritability analysis showed that The additive effect of genes primarily affect plant height, leaf width, petiole length, petiole width, and genetic additive effect and non-additive effect of the combined effect of strain rate, leaf length and area production. General combining ability showed that the female parent to be elected Suzhou Qing CMS and Xia WANG maintainer lines, male parent was selected Yin05, SHUIYUE rape and Ning ai Qing. According to effect size size of Specific combining ability, AiDan maintainer linexNing Ai Qing; AiDan maintainer linexSuigetsu rape; XiaWANG CMS×YIN05; XiaWANG maintainer linexSuigetsu rape、XiaWANG maintainer line×NingAiQing; SuzhouGreenCMS×Suigetsu rape; SuzhouGreen maintainer line×GreenYin0703Should be treated as a good hybrid combinations.2、The results showed that the contents of chlorophyll, soluble sugar, soluble protein, proline in CMS were significant lower than its maintainer, while the activities of three protective enzymes (SOD POD and CAT) were higher in CMS with the treatment of low temperature stress. The contents of chlorophyll and soluble protein decreased continuously in both CMS and its maintainer while, the contents of soluble sugar, praline and the activities of three protective enzymes (SOD POD and CAT) increased at first, then decreased under cold-stress. There is consistency in all stages of change between the CMS and its maintainer line and the reduce of the differences of Physiological and biochemical characteristics after processing, suggesting the resistance of cold stress of the non-heading Chinese cabbage OguCMS was improved greatly.3、The results of Optimizing ISSR molecular marker was that DNA concentration of40ng, Mg2+concentration of2.5mmol/L, dNTP concentration of0.2mmol/L, primer concentration of0.5mmol/L, Tag enzyme concentration of1.0U in the20ul reaction system, PCR amplification procedure was that94℃for5min,94℃denaturation1min,53℃annealing45s72℃extension1.5min,35cycles;72℃extension10min. The establishment of this system for future use of technology for non-heading Chinese cabbage germplasm classification, genetic mapping and gene location to build a technological basis. ISSR analysis of genetic markers of infertility results was U043numbers primer were screened in the75ISSR primers, it was repeatedly found in the male sterile line and maintainer line in the stability of specific amplified bands, and tested in the four hybrid generations were amplified Natiao stability of specific bands.