| The non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino) is a variant of Cruciferae Brassica cabbage subspecies, orginated in China with rich germplasm resources and nutritious, occupying an important status in vegetables, annual production and supply at Yangtze River. Mineral elements as an important part of the nutritional quality, have a close relationship with human health. However, mineral malnutrition is an important issue in the world, and information on the combining ability of mineral elements or the types of genetic effects is scarce, as it influences the selection of parents and breeding approaches, so we should improve nutritional quality of non-heading Chinese cabbage.This project collected 71 accessions non- heading Chinese cabbage inbred lines, use SSR marker to analyze the genetic diversity; The Ca, Fe, Mg and Zn contents of 71 accessions materials were determined by ICP-AE, analyzed the content level and distribution regularities of mineral elements in different materials, and analyzed the correlations among mineral elements and the correlations between mineral elements and other quality, and also did cluster analysis with 71 accessions inbred lines. Additional, we used hau CMS, eru CMS and their common maintainer line as female parent and 8 excellent and phenotypic different inbred lines as male parent,were designed with 3 ×8 incomplete diallel crosses to analyze the heterosis, combing ability, heritability and cytoplasmic effect of 4 main mineral elements(Ca, Fe, Mg and Zn). The main results were as follows:1. Screened 602 pairs of SSR primers, obtained 130 pairs with clear and stable, and amplified 317 polymorphic bands ranged from 80-500 bp, and the average of each pair primers amplified 2.44 polymorphic bands. C luster analysis were done by UPGMA method for 71 accessions non-heading Chinese cabbage inbred lines, Similarity coefficient of these accessions materials ranged from 0.57 to 0.99, 71 accessions materials were divided into five categories at the genetic similarity coefficient of 0.61 and each categorie also divided into several subgroups, morphology similar genetic material distance than more recent.2. Different elements contents in the same material content varies greatly with the order of Ca > Mg > Fe > Zn; and different materials have different mineral element content, the variation coefficients ranged from 14.25% to 33.23%, showing these materials exist significant genotypic differences.3. A extremely significant positive correlation existed between Ca and Mg(R=0.816, P〈0.01), and also a highly significant positive correlation existed between Mg and Zn(R=0.349, P<0.01). Significant positive correlations were found between Fe and VC and negatively correlated between Fe and crude fiber, Zn was positively correlated with soluble sugar.4. According to the contents of Ca, Fe, Mg and Zn, using the longest distance method for materials selection of Euclidean distance cluster analysis, in Dij2= 3.42 71 materials were divided into seven categories. Generally, Ca, Fe, Mg and Zn content in type IV and VI performe the best, while in group II and III performe the the worst.5. The HDH and MDH of Ca, Fe, Mg and Zn have corresponding positive crosses and heterosis is obvious, the three hybrid combinations of A1×C03, A2×C 03 and A2×C11 in the four elements expressed good performances in comprehensive with positive HDH and MDH.6. The cytoplasm positive effect of eru CMS is relatively high. The cytoplasmic effect of non-heading C hinese cabbage is affected by cytoplasmic origin and nuclear background.7. The parents C11 showed good general combiners for four mineral elements, the excellent parent to improve the four elements simultaneously and the best parents to modify Ca, Mg and Zn singlely. The parents C11, C03, C12 and C13 showed good general combiners for various mineral elements can be used as a good parent while improving the various elements. The cross A1×C03, A2×C11, B×C05 and B×C12 exhibits desirable significant specific combining ability effects for four mineral elements,and performance excellentiy comprehensive. The SCA and GCA have no direct relationship, and four mineral elements are mainly affected by non additive effect. |
PDF Full Text Request