Effect Of Light Intensity And Temperature On Datura Stramonium Linn

In this study, microscopical technique were used for compare the diffence of D.stramonium L. and D. stramonium var. tatual Torrey. root, stem, leaf anatomical structure.Plants Plants were grown under five levels of light intensity and four levels of tempreature inlight incubators, the morphologic characteristics, leaf anatomical structure, and pigment of D.stramonium L. and D. stramonium var. tatual Torrey. were studied. The results showed that:The root, stem, leaf anatomical structure of D. stramonium L. and D. stramonium var.tatual Torrey. were similar, but the first layer cells under the epidermis of D. stramonium var.tatual Torrey. stem, leaf-stalk, and venation were purple. They were incontinuity. Theincontinuity cells were also finded in the lower epidermis of D. stramonium var. tatual Torrey.leaf.Both plants grew best under13000and18000lx and had increased leaf and stembiomass with increased light intensity. Under the same light intensity, stem length, basal stemdiameter, blade number, bud length, leaf and stem biomass of D. stramonium var. tatualTorrey appeared higher than those of D. stramonium L., but most differences werestatistically insignificant. Leaf, palisade and spongy thickness and stomatal density and indexall increased with increasing light intensity. For both species, with increasing light intensitychlorophyll a, b and carotenoid concentrations in leaves showed open downward parabolictrends, chlorophyll a concentrations/chlorophyll b concentrations showed open upwardparabolic trends, chlorophyll concentrations/carotenoid concentrations decreased. Chlorophylla, b and carotenoid concentrations increased with increaseing light intensity in stems of D.stramonium var. tatual Torrey, but showed open downward parabolic trends in D.stramonium L.. Concentrations of anthocyanin, flavonoids, and total phenols of the twospecies increased with increasing light intensity.Both plants had increased stem length, blade number,leaf and stem biomass,reproductive growth with increased temperature, and decreased specific leaf area. The basalstem diameter of D. stramonium L. and D. stramonium var. tatual Torrey showed openupward parabolic trends. Leaf, palisade and spongy thickness of D. stramonium L. and D.stramonium var. tatual Torrey all decreased with increasing temperature, whereas palisadethickness/spongy thickness, stomatal density and index increased with increasing temperature.Chlorophyll a, b and carotenoid concentrations in leaves of D. stramonium L. increased with increasing temperature. The chlorophyll a concentrations/chlorophyll b concentrations,chlorophyll concentrations/carotenoid concentrations in leaves of D. stramonium L. andchlorophyll a, b and carotenoid concentrations, chlorophyll a concentrations/chlorophyll bconcentrations, chlorophyll concentrations/carotenoid concentrations in leaves of D.stramonium var. tatual Torrey showed open upward parabolic trends with increasingtemperature. For both species, Chlorophyll a, b and carotenoid concentrations in stemsincreased with increaseing temperature. Concentrations of anthocyanin in stems of D.stramonium L. and anthocyanin, flavonoids, and total phenols concentrations of D.stramonium var. tatual Torrey stems all showed open downward parabolic trends.Concentrations of flavonoids of D. stramonium L. stems increased with increasingtemperature. Concentrations of total phenols in stems of D. stramonium L. had insignificantdifference.Under the same light intensity and temperature, stomatal density and index, anthocyanin,flavonoids, and total phenols concentrations of D. stramonium var. tatual Torrey weresignificantly higher than those of D. stramonium L..