Evaluate Of Tea Germplasm Resources Of Camellia Sinensis Var. Assamica Cv. Jianghua

Camellia sinensis var. assamica cv. Jianghua was accounted the excellent local varieties in1987. It is the one of precious tea germplasm resources, which located in Nanling mountains Jianghua region Hunan province.This research is the first time using molecular marker technique and HPLC detection technology to evaluate Camellia sinensis var. assamica cv. Jianghua, results as fellows:1. The10pairs of ISSR primers were used to analyze the genetic polymorphism and genetic relationship of70C. sinensis var. assamica cv. Jianghua individuals. Totally174bands were generated with10ISSR primers, of which166(95.40%)were polymorphic, which indicated that the genetic diversity of’Jianghua’population was relatively high. The mean Nei ’s gene diversity (H) and the mean Shannon’s information index (l) of the tested tea germplasm were0.382,0.558, respectively. The genetic similarity coefficient ranged from0.44to0.84, averaging0.63,UPGMA cluster analysis of174DNA bands was made. The results showed that all the materials tested could be classified into6groups including5complex groups and1simple group.2.133Jianghua individuals was detected by both biochemical composition and catechin composition,which screening14high tea polyphenols (>34%) resources,9high free amino acid (>3%) resources,14high caffeine (>6%) resources,19high water extract (>48%) resources,11high ester catechins (>95%) resources,6high EGCG (>9%) resources.ISSR molecular marker result suggests that the genetic diversity of Jianghua tea germplasm was high and the genetic base was wide, select batch of high biochemical composition by detection, they show that Camellia sinensis var. assamica cv. Jianghua have high utility value in breeding tea deep processing.