Study On Cumulation Of Alkaloids Metabolism In Macleaya R. Br.Plants

Macleaya cordata (Willd)R. Br. and Macleaya microcarpa (Maxim) Fedde, which belongs to Papaveraceae, are tow species of Macleaya R. Br.plants. Modern plant chemical research shows that isoquinoline alkaloids are the main secondary metabolites in Macleaya R. Br. plants. Protopine (PRO), allocryptopine (ALL), sanguinarine (SAN) and chelerythrine (CHE) are the four main secondary metabolites in Macleaya R. Br. plants. Modern pharmacological studies show that the four alkaloids would be good alternatives to chemical antibiotics due to its effective anti-inflammatory activity.The content of the four alkaloids are dynamically changed in the growing Macleaya R. Br. plants. In order to synthetically utilize Macleaya R. Br. plants, it is necessary to research the regular pattern of alkaloids accumulate in the plants. In this study, the regular pattern of the contents of the four alkaloids and biomass will be explored in the aerial parts of Macleaya cordata (Willd)R. Br. In addition, in order to get the best habitats in a right time, which contain the highest content of the four alkaloids, we will study the regular pattern of the four alkaloids in different organs (roots, stems, leaves and fruits), different areas and different species (Macleaya cordata (Willd)R.Br. and Macleaya microcarpa (Maxim) Fedde). Moreover, the discrepancy of alkaloids metabolites in leveas of Macleaya cordata (Willd)R.Br. was studied based on HPLC tandem TOF-MS. The experimental results are showed as follows:1. The content of the four alkaloids in the aerial parts (leaves, stems, flowers and fruits) of Macleaya cordata (Willd) R. Br. was dynamically changed during different stages of growth. The contents of the four main alkaloids in different organs are different. Before fruit ripening, the two alkaloids, i.e., protopine and allocryptopine are accumulated mianly in the aerial parts of Macleaya cordata (Willd) R. Br. The best time for harvesting the leaves and stems of Macleaya cordata (Willd) R. Br. for the production of protopine and allocryptopine is the end of May. A rapid increase of the content of sanguinarine and chelerythrine in above-ground parts of Macleaya cordata (Willd) R. Br. was observed after the period of fruiting. The best time to harvest fruits of Macleaya cordata (Willd) R. Br. as raw material for the production of sanguinarine and chelerythrine is the end of August.2. Study about the regular pattern of the content of four alkaloids in roots, stems, leaves and fruits of Macleaya cordata (Willd) R. Br. and Macleaya microcarpa (Maxim) Fedde from different areas shows that the right time for harvesting of Macleaya cordata and Macleaya microcarpa (Maxim) Fedde is among August and September, but the time for harvest in different areas shows a little diffenece.3. The metabolites profile of the alkaloids in Macleaya cordata (Willd) R. Br. leaves during different grow stages was studied on the basis of HPLC-TOF-MS technology. The data was analyzed and20key variable was found by principal component analysis. These variable may cause the discrepancy of alkaloid metabolites in the leaves of Macleaya cordata (Willd) R. Br. during growing stages, in which five of them are identified. We preliminarily concluded that these compounds would be related with the biosynthesis of sanguinarme.