Study On The Anther Culture Of Weigela Florida Cv.’ Red Prince’

The technology of anther culture in vitro was an effective way for plant haploid breeding.lt was reported that the test of anther culture haploid plant had been successfully on Datura stramonium,Nicotiana tabacum L., Oryza sativa, Brassica oleracea var. acphala f. tricolor, Commelina communis L. and so on.According to the existing data,anther produced haploid plant by two means,one was pollen by similar embryos development process,formed pollen embryo,then haploid plant, such as Nicotiana tabacum, Datura stramonium;The other way was that pollen first formed callus,then induced haploid plant,such as Oryza sativa, Brassica oleracea var. acphala f. tricolor.The article through two Weigela florida cv.’Red Prince’development system in vitro to cytologic observation and statistical analysis,screened out factors of pollen starting division,suitable pollen development data,suitable inoculating data,suitable medium combination and other conditions.Thus for the effect on shortening breeding term,chromosome analysis and haploid using on genetic and breeding.The main contents and results of this study are as follows:1The selection of anther callus on inducing medium.The results showed that the medium1/3MS+6-BA(0.5mg/L)+NAA(2.0mg/L)+SUC(2%) is best,which is favorable to induce the anther callus on Weigela florida cv.’Red Prince’with temperature28℃,pH5.8,dark culture and24h per day.The average induction rate in callus is57.45percent.2The selection of pollen embryos on inducing medium.The results showed that the medium1/3MS+6-BA(2.0mg/L)+NAA(1.0mg/L)+SUC(1%) is best,which is favorable to induce the pollen embryos on Weigela florida cv.’Red Prince’with temperature28℃,pH5.8,dark culture and24h per day.The average induction rate in callus is15.15percent.3The suitable development data of pollen starting division.The suitable data of pollen starting division which developed callus or embryos was tetrad stage.The rate of callus and embryos was respectively53.2percent and6.73percent, all greatly higher than the uninucleate stage and the late uninucleate stage.4The suitable inoculating data of induction. The suitable inoculating data of pollen starting division which developed callus or embryos were all in six month at the beginning of flowering period and in seven month at the full flowering period,it was not suitable for eight month at the end of flowering period to inoculating culture. 5The influence of anther induction on lower temperature pretreatment.Put the anthers into the refrigerator of4℃,lower temperature pretreatment was processed in different days.The results showed that the highest induction rate of anther callus and embryos was the longest pretreatment(six days),which was41.98percent.The anther induction rate was only12.47percent without lower temperature pretreatment.6The main factors of suitable pollen starting division.The suitable factors for callus of pollen starting division were MS basic medium and sucrose.The lower the level of MS basic medium and sucrose,the higher the rate of callus; The suitable factors for embryos of pollen starting division were MS basic medium and6-BA.The lower the level of MS basic medium,the higher rate of embryos.the higher the level of6-BA,the higher the rate of embryos.