The Effects On Adsorption Of Zearalenone By β-D-glucans Extracted From Yeast Cell Wall And Its Derivative

ZEN is potentially harmful to both humans and animals health.Yeast glucan is one of the main components of Yeast cell wall. It shows many biological activities, such as immunomodulation, antitumor, antivirus, binding certain pathogens and their toxin etc. β-D-glucan was extracted from the yeast cell, and then was modificated by the chlorosulfonic acid-pyridine method. This experiment aims at discussing adsorption effect in vitro, and in vivo two experiments.In experiment1, yeast β-D-glucan was extracted from the yeast cell wall by enzyme-alkali method. The extracted products’s basic components were analyzed. Thin-layer chromatography (TLC) and FTIR spectrum analysis showed that D-mannose was not found in all of them.The extract was white flavourless and insoluble in water. And extracting rate was20.14%,87.36%of polysaccharide and3.23%of protein.In experiment2, this experiment investigated the synthesized conditions of sulfated yeast β-D-glucan (SYG) by the chlorosulfonic acid-pyridine method and discussed the efficacy on the adsorption of ZEA in vitro. The optimal reaction conditions were determined by a single-factorial experiment and orthogonal experiment with the index of degree of substitution (DS). The results indicated that the optimum conditions were as follows:reaction temperature of45℃, reaction time of2h and a CAS-Pyr ratio of1:6. Analysis by FTIR showed that:strong characteristic absorption peaks of S=O and C-O-S were observed at1259.56cm-1and808.12cm-1, respectively. Observation by scanning electron microscope showed that:larger specific surface area. Compared with β-D-glucan, SYG showed a very good efficacy on the adsorption of ZEA. Its affinity to ZEA was up to18.6528μg/mg SYG, which was greater than that of β-D-glucan with only2.2707μg/mg β-D-glucan.Yeast β-D-glucan and sulfated yeast β-D-glucan, an adsorbent for mixed feed, was added alone or simultaneously with a toxic ZEA and was evaluated for its ability to restore damages induced by ZEA. Thirty female KM mouse were equally divided into five groups of six mice per group as follows:blank control group of A, once perfusion ZEA (40mg/kg·bw) group of B, simultaneously perfusion ZEA (40mg/kg·bw) and adsorbent (Yeast, yeast β-D-glucan and sulfated yeast β-D-glucan) group of C, D, E. Forty-eight hours after treatment, blood samples were collected for serum biochemical parameters measurements. B group significantly increased AST and BUN, significantly reduce TP (p<0.05); AST, ALT, ALP and TBIL of C, D, E group was reduced compared with the B group; and the TP, ALB and CRE were increased, but significant different by each group (p>0.05). C, D, E group are no significant difference between the index (p>0.05). Thus, our data suggested that deleterious effects of ZEN, and yeast β-D-glucan and its derivatives could be reversed liver damage, but have less reliable.