Construction Of A Molecular Linkage Map In Lentinula Edodes And Mapping Quantitative Trait Loci Of Laccase Activity

In this study, the single spores W26and L6were used as parents, which derived from two cultivated mushroom strains WuXiang NO.1and L205respectively. We obtained a genetic segregation population with146spore monokaryons that originated from the hybrid W26×L6,as map population of Lentinula edodes. SSR, Indel and CAPS molecular matkers were used to analyse the map population for the construction of a molecular genetic linkage map in Lentinula edodes.38and17polymorphic primers were screened from151SSR and38Indel tested primers, respectively; while only one polymorphic primers were screened from83CAPS tested primers.56pairs primers screened in this study, perfecting the previous construction of the Lentinula edodes genetic linkage map. We generated a high-density framework map by JionMap(?)3.0software that contains571molecular markers and distributes11linkage groups. The length of this map was967cM and the average distance between two markers was1.87cM; the size of linkage groups ranged from22cM to233cM, its mean-size was87.9cM and the largest distance between markers was21cM.10molecular makers were linkaged to the genetic linkage map in this experiment,46molecular makers might be linkaged to telomeres or centromeres. Only one SSR molecular maker was linkaged to the genetic linkage map and9Indel molecular makers were linkaged to the genetic linkage map. The probability of2cM,5cM and10cM genetic distance cover one marker were80.1%,98.2%and99.9%in our high-density molecular genetic linkage map, respectively.Lentinula edodes laccase enzymatic activity was mapped as quantitative traits, we have identified various genomic regions that control them and that map to linkage groups. The results showed that,7laccase enzymatic activity QTLs mapped to linkage groups. qLac1and qLac2were mapped by using the total average of laccase enzymatic activity. qLacl mapped to about49.6cM of the LG3, R2=9.21%; qLac1mapped to about42.4cM of the LG6, R2=8.95%. The two QTLs got negative additive effect and the total R2of was18.16%. q Lac5-1and q Lac5-2were mapped by using the5d values of laccase enzymatic activity. q Lac5-1mapped to about40.6cM of the LG6, R2=9.73%; qLac5-2mapped to about48.4cM of the LG6, R2=9.04%. The two QTLs got negative additive effect and the total R2of was18.77%. q Lac15-1were mapped by using the15d values of laccase enzymatic activity. qLac15-1with positive additive effect mapped to about43.4 cM of the LG7, Rz=9.04%. q Lac20-1and q Lac20-2were mapped by using the20d values of laccase enzymatic activity. qLac20-1with positive additive effect mapped to about35.0cM of the LG7, R2=65.53%; qLac20-2with negative additive effect mapped to about57.7cM of the LG7, R2=17.63%.Using the SSR, CAPS and Indel molecular matkers perfected the Lentinula edodes genetic linkage map, and obtained a high-density molecular genetic linkage map of Lentinula edodes. Lentinula edodes laccase enzymatic activity related QTL mapping contributed to a fine-mapping of Lentinula edodes laccase genes. These also lay a good foundation for map-based cloning, quantitative traits positioning, and molecular genetic breeding in Lentinula edodes.