The Research On Technology Of Solid-state Fermentation On Feed Ingredients And It’s Feeding Effects

In recent years, the prices of feed ingredients continue to rise, the cost of livestock costs increased substantially, and it seriously affected the development of China’s feed industry and livestock. So promoting the feed efficiency and improving the feed quality is one of important researches in the feed industry currently. In this study, we research to the technology of the fermentation on the main feed ingredients corn and soybean of meal and it’s feed effects in growing and finishing pigs, the main results are as follows:(1) Through lots of screening and combined tests, determine the best combination of fermentation strains. we screened bacillus by Amylase activity、neutral and alkaline protease activity、antibacterial effect on E. coli and Staphylococcus as a primary screening,and got13strains of Bacillus. Then screened these13bacillus by solid-state fermentation and evaluated them by amylase activities、neutral protease activities、 reducing sugar content、TCA-N、CP digestibility in vitro. Finally choose Bsif, BS2、 NBS、Byml to be selected. We got3strains of lactobacillus with good production of acid which named EM、Zfl、Cfl, the total acidity of their liquid cultures were9.96%、8.51%、7.76%. Finally chose the Byml and EM as suitable strain combination by a mixed strain combination experiment.(2) We optimized the fermentation process by the single factor analysis and L943orthogonal design test based on pH、total acidity、CP digestibility in vitro, determined the optimum fermentation parameters were:fermentation period72h, temperature37℃, anticipate water to compare1:1(w/v), lactobacillus2%(v/w), bacillus3%(v/w), yeast3%(v/w). The feed ingredients fermented by probiotic through a solid-state fermentation, the pH can reach3.66or lower, the total acidity can reach5.80%, the crude protein digestibility in vitro can be increased to85.04%in theory.(3) We feed the Duroc-Landrace-Yorkshire piglets which weighed about25kg with the fermented feed. Compared with control group, the low addition feed group significantly improved the end average weight and average daily gain weight(P<0.05), and significantly reduced feed conversion ratio(P<0.05), reduced the diarrhea rate by52.7%, had little impact on morbidity; the high addition feed group reduced the diarrhea rate by36.4%,but the average daily feed intake, the average daily gain weight, feed conversion ratio and morbidity are no obvious difference(P>0.05).The albumin/globulin, albumin, serum urea nitrogen of the low addition feed group were significantly higher than the control group(P<0.05);the the total protein content of the high addition feed group was significantly higher than the control group(P<0.05),the alkaline phosphatase and blood glucose content were significantly lower than the control group(P<0.05). The other serum biochemical, immunological and anti-oxidation index had not significant difference.The low substitute feed group improved the CP’s displaying digestibility by3.68percentage point, the high substitute feed group improved it by2.57percentage point. The animal experiment prove that the solid-state fermentation can improve the quality of feed, promote the feed efficiency, improve animal growth performance and health status, reduce the diarrhea rate.