The Analysis Of Heterosis In Wheat By Using Dynamic Mitochondria Proteome

The research of molecular mechanism of heterosis in crops has a important significance in agricultural production, so many researchers have performed extensive research in rice, maize, wheat, rapessed and others. In the previous, however, majority of efforts had been focused on hybrid breeding, gene expression and transcription, isozyme analysis, epigenetic regulation, differentially expressed protein and so on, but at present the exact moleculer mechanism of heterosis is still unknown. This study is based on this, from the angle of the mitochondrial proteome to analyze the relations between differentially expressed mt-protein and heterosis.We used the2d,4d,6d old leaves as experimental material, analyzed and identified the differentially expressed proteins between hybrid and parents by using a combination of2-DE and MALDI TOF MS. The results indicated that there is a obvious difference in wheat leaf mitochondria proteome between hybrid and its parents, the number of the differentially expressed spots are22spots/2d,23spots/4d,33spots/6d, respectively. We also analyzed the expression pattern and the frequency of the differentially expressed proteins, we found four types:Additivety type (ADT), Parent di-type (PDT), High parent dominice type (HPDT) and Low parent dominance type (LPDT), the majority of them are partial to the male parental line6DL/6VS. In6d old leaves, the expression frequency of four models were15.2%(5/33),54.5%(18/33),24.2%(8/33) and6.1%(2/33).We choosed the mt-protein profiling of6days leaves as analysis object, and22spots of the33differentially expressed proteins were identified by using MALDI TOF MS. They involve in energy metabolism, cell structure constituent, protein transport and synthesis, stress reaction, secondary metabolism and other diverse biological functions. We predicted that there is a relation between the differentially expressed proteins and hybrid’s advantage traits, but the exact relation between the differentially expressed proteins and heterosis need further research.