The purpose of this study was to investigate the effects of copper and zinc protein chelate instead of inorganic trace mineral copper and zinc in different percentages (30%or100%) on immune organ index, blood parameters, intestinal morphology and mucosal immunity in Growth-Finishing Swine. The experiment included two parts:Experiment one, the first experiment was conducted to study the effects of copper protein chelate instead of inorganic trace mineral copper in different percentages (30%or100%) on immune organ index, blood parameters, intestinal morphology and mucosal immunity in Growth-Finishing Swine.101day-old48(Duroc×LandracexYorkshine) growth-finishing pigs were randomly allocated into3treatment groups with4replications of4pigs per replicate pen for each treatment group in68days in trial. The treatments were designed as follows:CUSO4group:basal diet with Cu from inorganic trace mineral copper;30%copper chelate group:basal diet with Cu from copper protein chelate instead of Cu from inorganic trace mineral copper in30percentage;100%copper chelate group:basal diet with Cu from copper protein chelate. The results showed that:1. The indexes of the liver, renal and spleen of30%or100%copper chelate group had increasing trend compared with the CUSO4group (P>0.05).2. The100%copper chelate group significantly increased LYM, HGB, HCT, MCH and MCHC compared with the CuSO4group (P<0.05), and the HGB of the100%copper chelate group than those in the30%copper chelate group (P<0.05); these other blood parameters of30%or100%copper chelate group had increasing trend compared with the CUSO4group (P>0.05).3. The30%or100%copper chelate group compared with the CUSO4group had no influence on the indexes of intestinal morphology (P>0.05).4. The100%copper chelate group compared with the CUSO4group increased the intraepithelial lymphocytes number of duodenum (P<0.05) and jejunum (P<0.01), and the intraepithelial lymphocytes number of duodenum of the100%copper chelate group than those in the the GuSO4group (P<0.05); the goblet cell number of duodenum and jejunum of30%or100%copper chelate group had increasing trend compared with the CuSO4group (P>0.05).5. The30%or100%copper chelate group compared with the CuSO4group significantly decreased IL-6level of jejunum (P<0.05); the IL-2level of duodenum and jejunum and the IL-6level of jejunum of30%or100%copper chelate group compared with the CuSO4group all had increasing trend (P>0.05).6. The30%or100%copper chelate group significantly decreased S-IgA level of jejunum compared with the CuSO4group (P<0.05); the S-IgA level of duodenum of30%or100%copper chelate group compared with the CuSO4group had increasing trend (P>0.05).Experiment two, the second experiment was conducted to study the effects of zinc protein chelate instead of inorganic trace mineral zinc in different percentages (30%or100%) on immune organ index, blood parameters, intestinal morphology and mucosal immunity in Growth-Finishing Swine.101day-old48(Duroc×Landrace×Yorkshine) growth-finishing pigs were randomly allocated into3treatment groups with4replications of4pigs per replicate pen for each treatment group in68days in trial. The treatments were designed as follows:ZnSO4group:basal diet with Zn from inorganic trace mineral zinc;30%zinc chelate group:basal diet with Zn from zinc protein chelate instead of Zn from inorganic trace mineral zinc in30percentage;100%zinc chelate group:basal diet with Zn from zinc protein chelate. The results showed that:1. The30%or100%zinc chelate group compared with the ZnSO4group very significantly increased the index of liver (P<0.01); the100%zinc chelate group compared with the ZnSO4group very significantly increased the index of renal (P<0.01); the30%or100%zinc chelate group had no influence on the index of spleen compared with the ZnSO4group (P>0.05).2. The30%or100%zinc chelate group had no influence on the blood parameters compared with the ZnSO4group (P>0.05).3. The30%or100%zinc chelate group had no influence on the indexes of intestinal morphology compared with the ZnSO4group (P>0.05).4. The100%zinc chelate group significantly increased the intraepithelial lymphocytes number and goblet cell number of jejunum (P<0.05); the intraepithelial lymphocytes number and goblet cell number of duodenum of the30%or100%zinc chelate group had increasing trend compared with the ZnSO4group (P>0.05).5. The IL-2level of duodenum and jejunum and the IL-6level of duodenum of the30%or100%zinc chelate group compared with the ZnSO4group had increasing trend(P>0.05); the IL-6level of jejunum of the30%or100%zinc chelate group compared with the ZnSO4group had decreasing trend (P>0.05).6. The S-IgA level of duodenum of the30%or100%zinc chelate group compared with the the ZnSO4group had increasing trend, but they didn’t reach significant level (P>0.05); the S-IgA level of jejunum had decreasing trend, but they didn’t reach significant level (P>0.05).According to the above results, copper and zinc protein chelate could increase the immunity of growth-finishing swine, and copper protein chelate insteaded of inorganic trace mineral in100percentage was better than in30percentage. |