The Research On Purification And Biological Activity Polysaccharide From Theplatycodon

Platycodon grandiflorum are widely distributed in the majority of our region and KoreanPeninsula, Japan and Eastern Siberia. It is a medicinal and edible plant. It has been developedin a number of ways to make good use of its value. This paper selects the Platycodongrandiflorum from Inner Mongolia Chifeng area as the test material, to provide the basic datafor further development by hot-water extraction, purification and activity research to the rootpolysaccharide.Before Polysaccharide extraction, we need drying, grinding, petroleum ether degreasingtreatment. Then by hot-water extraction, filtering, removal of protein through the membrane,ultrafiltration, alcohol precipitation, freeze drying, we can obtain coarse polysaccharide. Thenwe separated the obtained polysaccharide of Platycodon, so as to abtain three components:the molecular weight of less than72.67%of the6KDa （PPIIa）,24%of the molecular weightof between6KDa and10KDa （PPIIb）,3.33%of the molecular weight greater than10KDa（PPIIc）.Removing protein process for the coarse polysaccharide solution, we use5methods tocompare the removing protein: the method of Sevag, hydrochloric acid, acetic acid （TCA）method, enzymatic method, enzyme method and Sevag method. The way that we can abtainthe most suitable Platycodon polysaccharide is to use the protein clearance andpolysaccharide loss rate as an index, the method of trichloroacetic acid （TCA）. This methodremoval rate is89.35%, but the polysaccharide loss rate is only8.55%.We selected DEAE-52cellulose column chromatography and SephadexG-100chromatography to separate and purify the Platycodon polysaccharide PP II A and PP II B,using distilled water and three salt concentration gradient elution to purify DEAE-52cellulose.Finally we only get the water fractions of PP II A as PPIII and the water fractions of PP II Bas PPIV. The two components are respectively by using SephadexG-100columnchromatography for further purification, so we can obtain the single peak.Separately using the bacteria （Escherichia coli, Staphylococcus aureus, Bacillus subtilis）,fungal （aspergillus, Penicillium, Aspergillus niger）, we study the antibacterial test and theanti-oxidation activity of (O^2-·、·OH,removal capacity). The results showed that the proteinseparation and purification of antibacterial activity had little effect, the ability ofpolysaccharide inhibition of fungal is stronger than in bacterial inhibition. In a certainconcentration range, the antioxidative activity of polysaccharides is proportional to theconcentration of the coarse polysaccharide.The mouse entity anti fatigue activity experimentsshow that Platycodon polysaccharides significantly improve the swimming time of the mouse.Serum urea nitrogen, blood lactate, glycogen index showed Platycodon polysaccharides has obvious anti fatigue activity.