A GC-MS Analytical Study On The Metabonomics Of Added Pigments For The Chickens And Their Products

Now the detection mean for the pigments in the animals is few, and the detection sensitivity is low. As an emerging subject, metabonomics is playing an important role in the food safety detection. Metabonomics technology is used to do a systematic research to discover the endogenous metabolites changes in the animals which are caused by the natural pigments and the synthetic ones and look for some biomarkers. With the help of metabonomics, we would indicate the tracing source of the pigments in the poultry products, which would provide scientific basis for evaluating the pigments safety of the poultry products.In this dissertation, we chose the natural pigment-lutein and the synthetic pigment-sudan IV as the experiment subjects, and established a sample preparation method and Gas Chromatography-Mass Spectrometry (GC-MS) analytical method which would apply to the metabonomics study. Combining the GC-MS analytical method with the principal components analysis (PCA), this paper studied the endogenous metabolites changes in the chickens and their products which were caused by the two different pigments respectively. The results showed that when different concentrations of lutein and sudan IV were added to the feed, the metabolizes of the chickens’ serum, egg, chicken, liver and feces all took some changes. The primary metabolites that had significant changes were some amino acids, fatty acids, organic acids and carbohydrates. The concentration of palmitoleic acid, linoleic acid, oleic acid and stearic acid of the chickens’liver and feces was more and more high when the concentration of lutein was higher than before; While the concentration of sudan IV was higher than before, the concentration of malonic acid of the chickens’chicken and liver was more and more high, but the concentration of methyl-beta-D-galactopyranoside was more and more low. All of these substances would be identified as the potential biomarkers of the endogenous metabolism changes in the chickens which were caused by lutein and sudan IV.