Research On In Vitro Culture And Plantlet Regeneration Of Vaccinium Uliginosum

Vaccinium uliginosum were Ericaceae of the Vaccinium SPP. And the vaccinium uliginosum known as persimmon and Blueberry were shrub fruit trees. The fruit contained anthocyanin and flavonoids and could prevent and cure a high blood pressure disease.At the same time they could improve capillary permeability and relieve physical fatigue and they known as the "king of the berries". Vaccinium uliginosum was approved one of the five human health food by United Nations Food and Agriculture Organization. Planting area and production of cranberry were increasing in ten years all over the world. Vaccinium industry had rapid development in twenty years in China and became one of the most potential the fruit. Experiment material was the best one and study on the effects of many factors on plant regeneration with explant of stem and one node segments by tissue culture. And the factors included culture medium, hormone, pH and training time. Plant regeneration system of blueberries of Vaccinium uliginosum was developed.The results of the study were as follows:1Branches with indoor hydroponic culture could have green single bud about one week. The single buds were training as explant and the effect of elongation was obvious. Explant after10℃low temperature treatment could reduce the occurrence of browning and made the explant in exuberant growth condition and increased the survival rate of the buds.2The pollution rate of stems as explants was35%in explant of disinfection process. Young green bud was growing in one week. The pollution rate of single bud as explants was11.67%and it was less than the pollution rate of stems. A few of single bud were browning but its elongation growth faster.3The best growth medium of stem-segment with single bud is WPM,1.5mg/L ZT,0.3mg/L NAA,30g/L sucrose,8g/L AGAR. Optimum culture condition is pH5.4, the time of light8h/d, and the culture temperature is25/15℃(day/night). The best growth medium of single buds is WPM,2.0mg/L ZT,0.5mg/L NAA,25g/L sucrose,8g/L AGAR. Optimum culture condition is pH4.8, the time of light8h/d, and the culture temperature is25/15℃(day/night).4The single buds were training as explant and the effect was obvious and the cluster bud was wellgrown. The best growth medium of bud proliferation is WPM,1.5mg/L ZT,1.5mg/L6-BA, 15g/L sucrose and8g/L AGAR. The best times of secondary culture is three. Optimum culture condition is pH5.4, the time of light8h/d, and the culture temperature is25/15℃(day/night).5The single buds was training as explant with nutritional growth and medium proliferation.Then strong seedlings were cut from basal stem and inoculated to nutritional medium for30days and inoculated to rooting medium. The best rooting medium is1/2WPM,0.1mg/L IB A,1.5mg/L IAA,15g/L sucrose;8g/L AGAR. Optimum culture condition is pH5.6, the time of light set to8/day, and the culture temperature is25℃.