| In the experiment, my objects (Rana chensinensis) of study are from two different regions in our country.(Hunchun city in Jilin Province and Wuchang city in Heilongjiang Province). For17female frogs and14male frogs, I analyzed body measurements of the diversity of morphological characters and evaluated the index nutrition. Through those ways,1studied the genetic variations and genetic diversities of the wood frogs. And then, I probed the relationship between the genetic diversity and genetic differentiation of the two populations and the environment. I objectively assessed the germ plasm resource. Through analysis and correlation analysis, I found the main morphologic characters which affect the weight and the body length of the wood frog. The followings are the main results:1. During the growth spurt of the Rana chensinensis, along with the growth of the age of frogs, the frog body signs showed the growing trend. However the growing parameters of the male frogs and the female frogs are different. The appearances of the same aged frogs are obvious different, the female frogs’body forms are huge, and the weights are heavier.2. Because of the doubled influence of the ecological environment and the hereditary variation, in the body length and the posture and facial features respects, the frogs from the two regions are both different. Just the significant different parameters of the posture and facial features are different. And the female frogs from Hunchun City can spawn more. According to the coefficient of variation, I can ensure the difference between the two populations doesn’t reach the subspecies level. We can not identify them by the colors of the body surface. In order to identify them,we must do it with the help of body measurement.3. By means of regression analysis, I choose six female Rana chensinensis whose related coefficient of the morphological characters and weights attained the level of significance.(P<0.05) established the regression equation of body length (x1), head breadth(x2), the overall length of the hind legs (x3), body breadth (x4), number of the ovums (x5), weight of the egg mass/body weight (x6) to body weight (y). y=-17.285+0.019x5-60.014x6+0.023x3+0.042x1-0.095x2+0.022x4I choose three male Rana chensinensis whose related coefficient of the morphological characters and weights attained the level of significance.(P<0.01) established the regression equation of body weight (x1), head Iength(x2),shin length(x3) to body length (y). y=23.984+0.534x1+0.601x2+0.380x3. These establish the solid foundation of breeding the high quality Rana chensinensis.4.The nutrition constituent of the Rana chensinensis’muscle legs from two regions increase with age. There are few nutrition constituent differences between the frogs from the two regions. However, the female frogs’crude protein content, total amino acids and essential amino acid index are higher than the male frogs’. So3-year-old female frogs’taste is more delicious. The leg meat of the Rana chensinensis contains high protein, and it can be absorbed easily by human body. It has low fat content, its trophic value is very high. 5. The amino acids content from the two regions’Rana chensinensis are high and balanced. And there is no significant difference. Both of them are rich in high quality protein. In the muscle legs of Rana chensinensis from the two regions(Hunchun city in Jilin Province and Wuchang city in Heilongjiang Province), the the first restrictive amino acid is valine, the second restrictive amino acid is threonine. For EAAI of the Rana chensinensis from the two regions, the female frogs’is a little higher than the male frogs’. The EAAI of the Rana chensinensis from the Hunchun city in Jilin Province is higher than the EAAI of the Rana chensinensis from Wuchang city in Heilongjiang Province. These provide the theoretical basis for predicating the germ plasm resource and seed selection and breeding of the high quality varietals. |
PDF Full Text Request