Analysis Of The Bacterial Diversity And Isolation Identification Of Microorganisms From Akesu Saline-alkali In Xinjiang

This study based on degeneration of the gradient gel electrophoresis (Denaturing gradient gel electrophoresis, DGGE) of16S rDNA and cloning sequencing analysis the bacterial diversity and the dominant population in saline-alkaline soils of A-ke-su in Xinjiang, tried to find the relation between dominant population with environmental factors;Bacteria were isolated by using conventional culture-dependent method and investigated by means of phylogenetic analysis based on16S rRNA gene sequence comparisons.Evaluate the abilities of each medium recovery the soil microbial by the DGGE. In addition to a very few similarities has very big disparity, every culture medium each has its strong point, but16S rRNA gene sequences results that add growth factor for medium have more potential new classification.The result indicated that diversity of microbial of saline-alkaline soils in A-ke-su in Xinjiang was variety. A lot of groups of bacteria advantage bacterium group were found.29sequences belonged to the uncultivation of microorganism, other43sequence belonged to nine orders of bacteria, i.e. Myxococcales, Pseudomonadales, Rhizobiales, Bacillales, Burkholderiales, Actinomycetales, Oceanospirillales, Flavobacteriale, Alteromonadales. Canonical correlation analysis (CCA) result showed that microbial community structure and environmental factors are closely related in saline-alkaline soils.We isolated101bacterium strains perform a phylogenetic analysis based on16S rRNA gene sequences. Our results showed that101isolates belonging to31genera of10families in three phylogenetic groups (Actinobacteria, Firmicutes, Proteobacteria). The dominant population belonging to Bacillus (36.27%)、Streptomyces (10.8%)、 Micrococcus (6.9%)、Kocuria (6.9%) and Paracoccus (3.9%)。The phylogenetic distance matrix results suggested that there were obvious genetic divergences between most isolates and their closestly related type strains(16S rRNA gene sequence similarities ranged from97to100%),and that, out of101solates,at least5strainscould represent5potential new species within5characterized genera ().In conclusion, the results presented above showed that there are abundant bacterial diversity and a number of potential new taxa collected from the saline-alkaline soils of A-ke-su in Xinjiang and that these strains are important resources for bioactive compounds.In order to find some isolates exhibiting strong bioactivity to be further used in exploitation...