Analysis Of Wheat Relatives Resistance To Heterodera Avenae And H. Filipjevi

The cereal cyst nematode (CCN) is becoming one of the importantworldwide soil-borne pathogens, which has brought serious threat to wheat productionin13provinces of China, such as Henan, Hebei, Shanxi, Qinghai and so on, especiallyin Huanghuai wheat plant area in recent years. CCN has showed a trend of increasingyear by year because of wheat-maize(Zea mays L.)-wheat cropping systems, as wellas, agricultural machinery inter-district homework. Recently Heterodera avenae andH. filipjevi found in Henan province has caused great damage and yield loss andbecome a prominent issue in China’s wheat production.As we all know, the application of resistant or enduring varieties has become aneconomical and effective measure to prevent and control this disease. In fact, thelimited resistance resource can not meet the need of wheat breeding. Wheat relativesare important resources of multiple resistance genes to many biotic and abioticstresses, including CNN resistance genes. Using relative resistance index (RRI) asthe evaluation indicator, we screened resistant resources against H. filipjevi and H.avenae from more than20of wheat and its relatives after artificial inoculation. Threesets of common wheat (Triticum aestivum L. cv. Chinese Spring) chromosomeaddition lines with individual D. villosum chromosomes1V to7V, as well as eleven6VS translocation lines from different origins, were evaluated for CCN resistance.More stocks including Ae. speltoides、Ae. peregrina and Ae. geniculata additionallines were also been tested. Meanwhile, one set of D. villosum chromosome additionlines were test for H. avenae, Puyang population resistance and chromosomallocalization of resistance genes. The major research results were as follows:1. The resistance of more than20wheat and its relatives were tested and evaluatedagainst H. filipjevi and H. avenae in greenhouse. The results show that Roeogneria,Dasypyrum, Triticum boeoticum, Aegilops tauschii and Ae. speltoides were resistantto two pathogenic nematodes. In addition, T. durum, T. timopheevi and S. cerealewere good resistance resources of H. avenae. Totally speaking, there were morematerials resistant to H. avenae, Zhengzhou population than H. filipjevi, Xuchangpopulation in this study. 2. The result of identification of three sets of common wheat (Triticum aestivum)chromosome addition lines involving different D. villosum chromosomes and6VStranslocation lines from different origins indicated that Triticum aestivum-D. villosum6V chromosome addition line was highly resistant to CCN. However, thetranslocation lines6VS displayed various degrees of susceptibility. Based on thesefindings, CCN resistance was most probably related with chromosome6VL.3. By the research of resistance reaction of D. villosum additional line to H. avenae,Zhengzhou population and H. avenae, Puyang population, test analysis concluded thatDasypyrum villosum had high and stable resistance to two pathogenic nematodes, butthe background parent Chinese Spring(CS) had showed different reaction. Forinstance, CS was resistant to Zhengzhou population, but susceptible to Puyangpopulation. What is more, the resistance genes in D. villosum to Puyang populationmay on chromosomes2V、3V and6V respectively.4. Analysis of Ae. speltoides, Ae. peregrina and Ae. geniculata chromosome additionlines for H. filipjevi and H. avenae resistance, it was preliminarily presumed thatmany resistance genes of H. filipjevi exist in Aegilops. CS-Ae. speltoides SEchromosome addition line from Ae. speltoides was high resistance to H. filipjevi;resistance genes to H. filipjevi may on chromosomes3SV、4UVand6UVin Ae.peregrina; CCN resistance was probably related with chromosome7Ug、5Mgand2UginAe. peregrina. In order to locate the resistance genes accurately, many moretranslocation lines of Dasypyrum and Aegilops need to be identified.5. Based on these findings, multiple groups of data showed that Chinese Spring aswell-known special bread wheat was stable resistance to H. avenae Zhengzhoupopulation with RRI between0.70and0.75. Chinese Spring is not only a widely usedalien gene transfer tool, but also a potential CCN resistance resource.