Metabolism Of Green Tea And Tea Polyphenols In Chicken And Their Influence On Protein In Plasma

In this study a total of108broilers at age of40days were caged, and fed in the regular way. green tea powder of500mg/kg, tea polyphenols of300mg/kg, and EGCG of300mg/kg were respectively administered for grouped birds. Free tea catechins and catabolites in plasma and feces from all birds were respectively identified and quantified by high-performance liquid chromatography and by high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). Plasma protein abundence and composition were examined by SDS-PAGE. Our aim is to investigate the tea polyphenols and their metabolites in plasma and feces, and the effects of tea polyphenols on protein in plasma. The results are followed as after analysis:1. The green tea was analysed by Colorimetry and HPLC. The total content of tea polyphenols in green tea was19.17%, GA, GC, EGC, C, EC, EGCG, GCG, ECG. were2.22mg/g,4.76mg/g,22.63mg/g,17.43mg/g,5.53mg/g,53.52mg/g,1.76mg/g,18.41mg/g respectively2. The plasma was collected and analysed by LC-MS/MS. Pyrogallol acid (PA), GC,(-)-5-(3’,4’-dihydroxyphenyl)-γ-valerolactone(M6),C,EGCG,O-methyl-gallocatechin-O-glucuronides (O-Me-GC-O-GlcUA), epicatechin-glucuronides+H+(EC-GlcUA+H+)were identified in pasma from the green tea fed group. These components were also identified in the tea polyphenols fed group. EGCG, GCG, and O-Me-GC-O-GlcUA were identified in plasma from the birds administered EGCG. GA, O-methyl-catechin-O-sulphates (O-Me-C-O-S),O-methyl-gallocatechin-O-sulphates (O-Me-GC-O-S), EGCG, GCG, ECG, O-Me-GC-O-GlcUA, EC-GlcUA+H+were identified in feces from broilers administrated green tea. The feces from broilers administrated300mg/kg TP contained GA, EGCG, GCG, ECG, O-Me-C-O-S, O-Me-GC-O-S. The components identified in feces from broilers administrated EGCG group contained O-Me-C-O-S, GCG, EGCG, ECG. The metabolites in plasma contained O-methylated and glucuronide conjugates of catechin, but the feces contained O-methylated, sulphated, and glucuronide conjugates of catechin.3. The plasma was analysed by HPLC. The maximum concentrations of free catechins GC,(+)-C in plasma after administrated GT were20.21μ mol/L,76.99u mol/L respectively at4h, and the maximum concentrations of EGCG was34.28u mol/L at8h. The peak levels of GC and C in plasma from TP fed group.were40.35u mol/L,17.25μ mol/L respectively at4h, but EGCG in plasma dispeared between4h and8h. For EGCG group, EGCG decomposed into GCG partly between0h and1.5h, and then dispeared between4h and8h. The peak levels of O-Me-GC-O-GlcUA and EC-GlcUA+H+in plasma from TP fed group were1.13μmol/L,1.95μ mol/L respectively at4h. The maximum concentrations of O-Me-GC-O-GlcUA in plasma presented at4h after administrated EGCG. PA level in plasma from GT fed group was max at4h, and M6level in plasma from GT fed group reached max value at8h. For TP group, PA level in plasma reached max value at4h, and M6in plasma was not detected between Oh and1.5h after adimistrated TP. None of lysates in plasma from EGCG fed group were determined over the hour0-8h.4. The feces samples were analysed by HPLC. GA, EGCG, GCG, ECG in the feces reached the peak exceretion at4h after administrated GT and TP. For TP group. EGCG and ECG in the feces reached the max value at4h after ingestined EGCG. The metabolition in the feace from GT group, O-Me-GC-O-S reached maxmum at4h, O-Me-C-O-S and O-Me-GC-O-GlcUA disappeared between1.5h and4h, but EC-GlcUA+H+disappeared between4h and8h. For TP group,the peak exceretion of O-Me-C-O-S in the feces was at4h, O-Me-C-O-S in the fece was only determined, reached maxmum at4h after administered EGCG during the test.5. The GT and TP could improve the total protein in plasma at1.5h, but TP had better effect. Comparison with the control, the total protein level decreased at4h after administrated GT. The total protein concentration was significantly enchanced at4h after ingested TP and EGCG. However, the total protein level significantly decreased between4h and8h after administrated GT and EGCG when compared with the control.6. TP could enchance the protein band for67.5kd, and EGCG caused the disappearance of band for107.1kd after administrated at1.5h. The band of protein for67.5kd could be enchanced by TP and disapperared by EGCG after ingested at4h. The protein level was no significantly change in all samples. The green tea and its extraction could be responsible for improving the protein composition in plasma in4h.