Effects Of EGCG And ECG On Mammalian Reproductive Cell

EGCG is the most abundant Tea Polyphenols single material, it can be played effectively remove antioxidation and the role of free radicals. In this study, sperm abnormalities induced by cyclophosphamide as a positive control group, and observe whether EGCG itself the role of sperm abnormalities induced by different doses of EGCG. Studies in pig oocytes cultured added EGCG, EGCG observed whether to increase the effect of embryonic development, for other mammalian embryos in vitro to provide reference for. As the second largest component of catechin ECG would affect embryonic development in mammals to be studied. The study also through in vitro cultured mouse ECG added to the in vitro development of mouse embryos, and staining of embryos through the blastocyst morphology, in order to further improve the efficiency of mouse embryos in vitro and improve the quality of development to explore new ways and methods. The main findings are as follows:1. EGCG on cyclophosphamide-induced sperm abnormality of:In this study, sperm deformity rate in the CP group significantly higher than the negative control group (P<0.05). that successful model. CP+EGCG group, the dose of sperm abnormality rate was significantly lower than not only the CP group (P<0.05), but was significantly lower than the saline control group (P<0.05), that EGCG can inhibit sperm abnormalities in mice induced by CP, but also Sperm malformation rate decreased to below normal. The experiment also showed that EGCG role for mouse sperm deformity time differences exist, the first5weeks of different doses of EGCG group of sperm abnormality rate of less than4weeks of the test results, and the first5weeks testing different doses of EGCG Induction of anti-CP and4weeks of good test results.2. EGCG on porcine oocyte maturation:Different concentrations of EGCG maturation of pig embryos. EGCG can not effectively improve the cleavage rate, cleavage rate between experimental groups was not significant (P>0.05), add30μmol/L ECG cleavage rate slightly higher than the other groups. Showed an increasing trend. EGCG can promote the development of the blastocyst. the blastocyst rate between experimental groups was not significant (P>0.05), add10μmol/L EGCG high blastocyst rate slightly higher than the other groups. Different concentrations of EGCG on the maturation of pig embryos. the maturation rate in each experimental group were not significantly different (P>0.05), add10μmol/L EGCG was slightly higher than other groups, showing an increasing trend. The above data shows that. in the pig oocyte culture system to EGCG, the development of pig embryos in vitro can pus.3. ECG of mouse oocytes in vitro embryonic development:Different concentrations of ECG maturation of mouse embryos, ECG, and can not effectively improve the cleavage rate, cleavage rate between experimental groups was not significant (P>0.05), add30μmol/L ECG cleavage rate slightly higher than the other groups. ECG can improve the blastocyst rate, blastocyst embryos can promote the development, but no significant difference. ECG can effectively increase the cell number, add10μmol/L ECG was significantly higher than the control cells, and30μmol/L group (P<0.05), but with20μmol/L was no statistical difference. The above data shows that, in the mouse oocyte culture system to add10μmol/L and20μmol/LECG, the development of mouse embryos in vitro significantly promoted.