Optimization Of Fermentation Sesame Meal Conditions And Antionxidant Activity Analysis Of The Sesame Peptide

Sesame seed meal nutrition is rich and amino acid combination is comprehensive. Butsesame seed meal contains a large number of phytic acid and tannins, limiting its wideapplication in livestock diets. Microbial fermentation solve a lot of drawbacks anddisadvantages of the physical, chemical and the enzyme preparation method in thedegradation of anti-nutritional factors and the production of bioactive peptides, which isfurther practical significance for development and utilization of sesame meal In this study,we researched forage quality of fermentation improved sesame, sesame peptidespurification and its antioxidant activity. the results were as follows:1Study on sesame meal forage quality fermentation improved. Bacillus subtilis,lactic acid bacteria and yeasts were choose o reduce phytic acid and volatile base nitrogencontent, enhance the crude protein, soluble protein and other useful components in thefermented sesame meal through the single-factor experiment and L9(34) orthogonalexperiment optimizing of microbial fermentation conditions. The results showed thatoptimal fermentation conditions were as follows: the ratio of material to water was (W:V)1:0.8, R-02and KG-109mixed fermentation and the temperature was30℃, R-02andKG-109vaccination with ratio was2:1, inoculation quantity is8%, fermentation time was10d. Under the optimal fermentation that phytic acid content was0.08%, phytic aciddegradation rate was86.21%, the protein content was49.85%, acid soluble protein was9.07%, total volatile basic nitrogen was207.55mg·100g-1.2Study on isolation and purification of sesame peptides. Sesame peptide extractionconditions were optimized by single factor test and the peptide content of the crudeextracts of sesame peptides were regarded as an index. The results indicated that theoptimum extraction conditions of the material to water ratio was1:6, soaking extraction for15min in30℃. Crude extracts of sesame peptides were isolated and purified by SephadexG-15and got three different molecular weight peptides, which were determined as threepeptides, peptides and peptide.3Studies on the antioxidative activity of sesame peptides. In vitro antioxidant weredetermined the clearance activity of the sesame peptides on DPPH using DPPH method,scavenging free radical activity of the sesame peptides on the hydroxy using salicylic acidmethod, the total reduction ability of the sesame peptides using the method of ferricreducing ability. To determined antioxidant activity experiments in vivo by intragastriclavage tests,48healthy Kunming mice (4weeks age) were randomly divided four groups (control group, low dose group, middle dose group and high dose group),12mice pergroup. Mice were given saline as normal control,0.1g/kg·d,0.2g/kg·d and0.4g/kg·d ofsesame peptide for continuous intragastric28d, respectively. On the day of last treatment,mice were sacrificed to obtain blood and livers, and determined weight of organ, MDA,SOD, GSH-PX and CAT. The results show that the DPPH scavenging activity of1mg/mLthe extracting of sesame peptide,3peptide and4peptide were all90%, whereas1mg/mL6peptide was only80%; the OH scavenging activity of0.6mg/mL3peptide and4peptidewere all100%, and0.6mg/mL the extracting of sesame peptide and6peptide were all90%;the reducing powers of4mg/mL the extracting of sesame peptide,4and6peptide, and2mg/mL3peptide were corresponded to0.5mg/mL glutathione. After feeding6peptide,compared to control group, the difference of body and organ weight was not significant(P>0.05). Compared to control group, high dose group and low dose group can beobserved that6peptide can effectively reduce the content of MDA in serum and liver, andcan significantly improve the liver SOD, GSH-PX and CAT activity, and show adose-dependent. These results demonstrated the sesame peptide has stronger reducingactivity and the ability of scavenging DPPH and OH; and the antioxidative were enhancedwith sesame peptide molecular weight reduced.